AB243690

Human DKK4 ELISA Kit

Recombinant
SimpleStep
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Human DKK4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DKK4 with a sensitivity of 5.17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

sELISA

Supplier Data

Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK4 concentration was determined to be 61.9 pg/mL with a range of ND – 97.5 pg/mL. ND = not detected, below the range of the assay.

sELISA

Supplier Data

Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

Human DKK4 Standard Curve Comparison.

Standard Curve comparison between human DKK4 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

Serum from eight individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK4 concentration was determined to be 43.0 pg/mL with a range of ND – 113.2 pg/mL. ND = not detected, below the range of the assay.

sELISA

Supplier Data

Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

Interpolated concentrations of native DKK4 in human SW480 cell culture supernatant samples.

The concentrations of DKK4 were measured in duplicates, interpolated from the DKK4 standard curves and corrected for sample dilution. Undiluted samples are as follows : SW480 supernatant 100 %. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK4 concentration was determined to be 1,245 pg/mL in SW480 supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

Example of human DKK4 standard curve in Sample Diluent NS.

The DKK4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 5.17 pg/mL

검출 범위

21.88 - 1400 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human DKK4 ELISA Kit (ab243690) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DKK4 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DKK4 with 5.17 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "103 - 115 %", "average": "= 110" }, { "sample": "Serum", "range": "82 - 86 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "87 - 98 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "95 - 105 %", "average": "= 99" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Dickkopf-related protein 4 (DKK4) acts as an inhibitor of the Wnt signaling pathway which affects cell fate proliferation and migration. Known aliases for DKK4 include DKK-4 and DKK-4 protein. It possesses a molecular mass of around 27 kDa. DKK4 mainly expresses in the skin gastrointestinal tract and placenta indicating roles in these tissues and systems. Its presence suggests involvement in processes related to epithelial and stromal interactions.

Biological function summary

DKK4 participates in the regulation of embryonic development and tissue homeostasis. It functions as a part of the larger Dickkopf protein family which modulates Wnt signaling by binding to LRP5/6 co-receptors. Through this interaction DKK4 manages the balance between cell growth and apoptosis important in both early development and adult tissue maintenance. DKK4 presence is often necessary to modulate signals that govern differentiation and proliferation in complex tissue systems.

Pathways

DKK4 predominantly interacts within the Wnt signaling pathway. This pathway is critical for regulating gene expression involved in stem cell maintenance and differentiation. DKK4 acts by interacting with proteins such as LRP6 and Kremen thereby inhibiting Wnt-induced processes. Through modulation of these factors DKK4 ensures the fine-tuning of signaling cascades necessary for maintaining cellular equilibrium under physiological conditions.

Research associates DKK4 with malignant melanoma and colorectal cancer. These associations arise due to DKK4's role in modulating Wnt signaling which can drive oncogenic processes if dysregulated. In melanoma DKK4 can affect tumor cell proliferation by interacting with proteins like beta-catenin. In colorectal cancer abnormal expression of DKK4 might disrupt normal signaling balance further implicating it in tumor growth and progression. These connections highlight DKK4's potential as both a biomarker and a therapeutic target.

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제품 프로토콜

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타겟 정보

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).

See full target information Dickkopf-related protein 4

대체 명칭 보기

Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, DKK4

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human DKK4 ELISA Kit

Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

Sandwich ELISA - Human DKK4 ELISA Kit (AB243690)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise