AB246530

Human DMP1 ELISA Kit

Recombinant
SimpleStep
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Human DMP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DMP1 with a sensitivity of 22.58 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Sandwich ELISA - Human DMP1 ELISA Kit (AB246530)

sELISA

Supplier Data

Sandwich ELISA - Human DMP1 ELISA Kit (AB246530)

Example of human DMP1 standard curve in Sample Diluent 75BS.

The DMP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human DMP1 ELISA Kit (AB246530)

Interpolated concentrations of spiked DMP1 in human serum, plasma (citrate), and PBMC cell culture supernatant samples.

The concentrations of DMP1 were measured in duplicates, interpolated from the DMP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, and PBMC supernatant 50%. The interpolated values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human DMP1 ELISA Kit (AB246530)

Example of human DMP1 standard curve in Sample Diluent NS.

The DMP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 22.58 pg/mL

검출 범위

171.875 - 11000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human DMP1 ELISA Kit (ab246530) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DMP1 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DMP1 with 22.58 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 120 %", "average": "= 108" }, { "sample": "Serum", "range": "82 - 84 %", "average": "= 83" }, { "sample": "Citrate plasma", "range": "83 - 86 %", "average": "= 84" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein DMP1 also known as Dentin Matrix Protein 1 is part of the SIBLING (Small Integrin-Binding Ligand N-linked Glycoprotein) family and plays an important role in bone and tooth mineralization. It is encoded by the DMP1 gene and has a molecular weight of around 52 kDa. DMP1 localizes mainly to the extracellular matrix of bone and dentin and is highly expressed in osteoblasts odontoblasts and hypertrophic chondrocytes. This protein regulates the formation of hydroxyapatite a critical mineral component in the formation of hard tissues.

Biological function summary

The protein DMP1 is essential for phosphate homeostasis skeletal development and mineralization processes. It acts as a nucleator in the deposition of calcium phosphate minerals. As part of a protein complex DMP1 interacts with other extracellular matrix proteins influencing their functions. DMP1 plays an important role in the maturation of bone matrix and dentin effectively mediating the transformation of mesenchymal stem cells into osteoblasts and odontoblasts. It is also implicated in the regulation of gene expression linked to mineralization.

Pathways

DMP1 is closely involved in the bone mineralization and FGF (Fibroblast Growth Factor) signaling pathways. These pathways are important for maintaining bone density and controlling phosphate metabolism. DMP1 interacts with other proteins like PHEX (Phosphate Regulating Endopeptidase Homolog X-Linked) and MEPE (Matrix Extracellular Phosphoglycoprotein) within these pathways to facilitate proper mineralization of bone and teeth. Furthermore DMP1 functions alongside other SIBLING family proteins to modulate mineralization through their collective roles in the matrix.

DMP1 mutations or dysfunctions lead to conditions such as autosomal recessive hypophosphatemic rickets and osteomalacia. These conditions result from defective phosphate metabolism affecting skeletal mineralization and causing brittle bones and dental anomalies. In these scenarios DMP1's relationship with PHEX becomes evident as both proteins work together to regulate phosphate levels. Disruption in their function can significantly alter mineralization balance highlighting the necessity of DMP1 in maintaining skeletal and dental health.

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제품 프로토콜

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타겟 정보

May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.

See full target information DMP1

대체 명칭 보기

Dentin matrix acidic phosphoprotein 1, DMP-1, Dentin matrix protein 1, DMP1

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Product promise

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Human DMP1 ELISA Kit

Sandwich ELISA - Human DMP1 ELISA Kit (AB246530)

Sandwich ELISA - Human DMP1 ELISA Kit (AB246530)

Sandwich ELISA - Human DMP1 ELISA Kit (AB246530)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise