AB246524

Human ECM1 ELISA Kit

Name: Human ECM1 ELISA Kit
Brand: Abcam Limited
SKU: AB246524
Rating: 4 (1 reviews)

Recombinant
SimpleStep
각 태그의 뜻

(1 리뷰)

(2 제품이 사용된 논문 )

Human ECM1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ECM1 with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

4 이미지

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

sELISA

Supplier Data

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Interpolated concentrations of native ECM1 in cell culture supernatant.

The concentrations of ECM1 were measured in duplicate, interpolated from the ECM1 standard curves and corrected for sample dilution. Undiluted samples are as follows : MDA-MB-435S supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 141 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 2862 ng/mL with a range of 1028 – 4859 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Interpolated concentrations of native ECM1 in human serum, plasma.

The concentrations of ECM1 were measured in duplicates, interpolated from the ECM1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1000, plasma (citrate) 1 : 2000, plasma (EDTA) 1 : 2000, and plasma (heparin) 1 : 1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 4064 ng/mL in serum, 9512 ng/mL in plasma (citrate), 10307 ng/mL in plasma (EDTA), and 2989 ng/ml in plasma heparin.

sELISA

Supplier Data

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Example of human ECM1 standard curve in Sample Diluent NS.

The ECM1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 36 pg/mL

검출 범위

62.5 - 4000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human ECM1 ELISA Kit (ab246524) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ECM1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ECM1 with 36 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 104 %", "average": "= 101" }, { "sample": "Serum", "range": "104 - 132 %", "average": "= 114" }, { "sample": "EDTA Plasma", "range": "100 - 112 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "100 - 109 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "104 - 113 %", "average": "= 109" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab246524-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB246524-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB246524-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB246524-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB246524-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB246524-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB246524-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB246524-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ECM1 Lyophilized Recombinant Protein", "number":"AB246524-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ECM1 Capture Antibody", "number":"AB246524-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ECM1 Detector Antibody", "number":"AB246524-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Extracellular matrix protein 1 often called ECM1 is a matrix protein involved in various mechanical functions in the body. This protein weighs approximately 61 kDa and is widely expressed in human tissues including skin bone lung and brain. ECM1 also goes by the name H3300 vector used in some experimental contexts. Being an extracellular matrix (ECM) component ECM1 plays roles in tissue organization influencing matrix proteins' structural integrity.

Biological function summary

ECM1 contributes to cell-matrix interactions and regulates various cell behaviors such as adhesion proliferation and differentiation. It does this by binding to other ECM proteins and is known not to be part of a specific complex. ECM1 facilitates communication between the cellular environment and matrix thereby supporting the architecture and dynamics of tissues. It modulates angiogenesis linking it with processes like wound healing and vascular development.

Pathways

ECM1 functions within the context of key signaling routes such as the PI3K/AKT pathway known for roles in cell growth and survival. ECM1 interacts with other proteins including matrix metalloproteinases contributing to matrix remodeling activities important for cellular migration and invasion. Cumulatively these interactions highlight ECM1’s place in connective tissue organization and regenerative processes.

ECM1’s dysregulation links it with lipoid proteinosis and skin conditions like Atopic Dermatitis. Imbalances in ECM1 expression or function can lead to abnormal ECM assembly influencing these diseases' development. Moreover abnormalities in ECM1 can alter the behavior of related proteins such as collagen impacting skin integrity and permeability. Understanding ECM1’s role could provide insights into therapeutic strategies for these conditions.

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제품 프로토콜

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타겟 정보

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

See full target information Extracellular matrix protein 1

대체 명칭 보기

Extracellular matrix protein 1, Secretory component p85, ECM1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of extracellular vesicles 14:e70089 PubMed40545963

2025

Extracellular Vesicle Proteome Analysis Improves Diagnosis of Recurrence in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Yong Hyon,Min Woo Kim,Kyung-A Hyun,Yeji Yang,Seongmin Ha,Jee Ye Kim,Young Kim,Sunyoung Park,Hogyeong Gawk,Heaji Lee,Suji Lee,Sol Moon,Eun Hee Han,Jin Young Kim,Ji Yeong Yang,Hyo-Il Jung,Seung Il Kim,Young-Ho Chung

Biomedicines 9: PubMed34440227

2021

Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Del Amo,Arantza Perez-Valle,Miguel Perez-Garrastachu,Ines Jauregui,Noelia Andollo,Jon Arluzea,Pedro Guerrero,Koro de la Caba,Isabel Andia

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human ECM1 ELISA Kit

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise