AB269552

Human EFEMP1 ELISA Kit (Fibulin-3)

Recombinant
SimpleStep
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Human EFEMP1 ELISA Kit (Fibulin-3) is a single-wash 90-min Simplestep used to quantify Human EFEMP1 (Fibulin-3) with a sensitivity of 42 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

3 이미지

Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)

sELISA

Supplier Data

Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)

Interpolated concentrations of native EFEMP1 in human CSF.

The concentrations of EFEMP1 were measured in duplicates, interpolated from the EFEMP1 standard curves and corrected for sample dilution; Undiluted CSF is 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EFEMP1 concentration was determined to be 91 ng/ml.

sELISA

Supplier Data

Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)

Interpolated concentrations of native EFEMP1 in human serum and plasma samples.

The concentrations of EFEMP1 were measured in duplicates, interpolated from the EFEMP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 400, plasma (citrate) 1 : 800, plasma (heparin) 1 : 400, plasma (EDTA) 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EFEMP1 concentration was determined to be 742 ng/mL in serum, 1672 ng/mL in plasma (citrate), 1072 ng/mL in plasma (Heparin), and 997 ng/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)

Example of human EFEMP1 standard curve in Sample Diluent NS.

The EFEMP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 42 pg/mL

검출 범위

62.5 - 4000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human EFEMP1 ELISA Kit (Fibulin-3) (ab269552) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EFEMP1 (Fibulin-3) protein in serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EFEMP1 (Fibulin-3) with 42 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

EFEMP1, EGF-containing fibulin-like extracellular matrix protein 1, is a secreted extracellular glycoprotein in the fibulin family. It is a key regulator of cell-to-cell and cell-to-matrix adhesion. It is currently under investigation as a pharmacological target for a host of cancers. The antibodies for this kit were raised against recombinant protein representing the mature full-length human protein.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" } ]

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Recovery

[ { "sample": "Serum", "range": "90 - 106 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "93 - 110 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "88 - 104 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "86 - 105 %", "average": "= 97" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab269552-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB269552-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB269552-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB269552-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB269552-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB269552-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB269552-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB269552-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human EFEMP1 (Fibulin-3) Capture Antibody", "number":"AB269552-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human EFEMP1 (Fibulin-3) Lyophilized Recombinant Protein", "number":"AB269552-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human EFEMP1 (Fibulin-3) Detector Antibody", "number":"AB269552-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

EFEMP1 also known as Fibulin-3 is a member of the fibulin family involved in extracellular matrix (ECM) organization. The protein's mass is approximately 55 kDa. It is expressed in various tissues but researchers find it most common in the eye lung and arterial walls. As a secreted glycoprotein Fibulin-3 plays a vital role in maintaining structural integrity and elasticity in connective tissues.

Biological function summary

EFEMP1/Fibulin-3 participates in ECM modulation and cell signaling. It interacts with other ECM components such as fibronectin and collagen contributing to structural assembly and stability. Although not prominently part of multi-protein complexes Fibulin-3 influences cellular adhesion and migration impacting tissue development and repair mechanisms. Researchers observe its expression during embryogenesis suggesting a role in developmental processes.

Pathways

EFEMP1/Fibulin-3 integrates into matrix-related pathways impacting tissue architecture. It plays a role in the EGF (Epidermal Growth Factor) signaling pathway which is important for various cell survival and proliferation processes. Fibulin-3 exhibits functional relationships with proteins like fibronectin and integrins within these pathways highlighting its importance in mediating cell-matrix interactions and influencing signal transduction cascades.

EFEMP1/Fibulin-3 has notable connections with certain pathologies. Malfunctions in its expression or structure can relate to age-related macular degeneration and various forms of cancer including mesothelioma. In these diseases Fibulin-3 interacts with proteins such as matrix metalloproteinases which degrade extracellular matrix proteins thereby influencing tumor progression and ECM remodeling in affected tissues.

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제품 프로토콜

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타겟 정보

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.

See full target information EFEMP1

대체 명칭 보기

FBLN3, FBNL, EFEMP1, EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3, FIBL-3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 27:108930 PubMed38333700

2024

Serum proteome profiling reveals heparanase as a candidate biomarker for chronic thromboembolic pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxia Zhang,Meng Zhang,Hongwei Yang,Haobo Li,Shuangshuang Ma,Linfeng Xi,Yishan Li,Xincheng Li,Zhihui Fu,Zhu Zhang,Shuai Zhang,Qian Gao,Qiang Huang,Jun Wan,Wanmu Xie,Jifeng Li,Peiran Yang,Zhenguo Zhai

Journal of experimental & clinical cancer research : CR 42:328 PubMed38031171

2023

Unveiling the role of osteosarcoma-derived secretome in premetastatic lung remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Sara F F Almeida,Liliana Santos,Gabriela Sampaio-Ribeiro,Hugo R S Ferreira,Nuno Lima,Rui Caetano,Mónica Abreu,Mónica Zuzarte,Ana Sofia Ribeiro,Artur Paiva,Tânia Martins-Marques,Paulo Teixeira,Rui Almeida,José Manuel Casanova,Henrique Girão,Antero J Abrunhosa,Célia M Gomes

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human EFEMP1 ELISA Kit (Fibulin-3)

Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)

Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)

Sandwich ELISA - Human EFEMP1 ELISA Kit (Fibulin-3) (AB269552)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise