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AB100504

Human EGF ELISA Kit

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(3 제품이 사용된 논문 )

Human EGF ELISA Kit is a Sandwich ELISA for the measurement of Human EGF in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human EGF ELISA Kit (AB100504)
  • sELISA

Lab

Sandwich ELISA - Human EGF ELISA Kit (AB100504)

Standard curve in Diluent B : mean of duplicates (+/-SD)

Sandwich ELISA - Human EGF ELISA Kit (AB100504)
  • sELISA

Lab

Sandwich ELISA - Human EGF ELISA Kit (AB100504)

Standard curve in Diluent A : mean of duplicates (+/-SD)

Sandwich ELISA - Human EGF ELISA Kit (AB100504)
  • sELISA

Lab

Sandwich ELISA - Human EGF ELISA Kit (AB100504)

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 1 pg/mL

검출 범위

0.82 - 200 pg/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human EGF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 106 %", "average": "= 95.52" }, { "sample": "Serum", "range": "83 - 104 %", "average": "= 94.52" }, { "sample": "Plasma", "range": "84 - 105 %", "average": "= 93.76" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100504-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100504-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100504-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100504-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"EGF Microplate (12 strips x 8 wells)", "number":"AB100504-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100504-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100504-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB100504-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human EGF", "number":"AB100504-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human EGF Standard (lyophilized)", "number":"AB100504-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.
Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.
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제품 프로토콜

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타겟 정보

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed : 10964941).
See full target information EGF

대체 명칭 보기

Pro-epidermal growth factor, EGF
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of stem cells 13:93-103 PubMed32114740

2020

The Overexpression of Epidermal Growth Factor (EGF) in HaCaT Cells Promotes the Proliferation, Migration, Invasion and Transdifferentiation to Epidermal Stem Cell Immunophenotyping of Adipose-Derived Stem Cells (ADSCs).

Applications

Unspecified application

Species

Unspecified reactive species

Yueping Mao,Jianchi Ma,Yue Xia,Xiaoyuan Xie

Cancer research 79:4057-4071 PubMed31292163

2019

ID1 Is Critical for Tumorigenesis and Regulates Chemoresistance in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rohit Sachdeva,Megan Wu,Sandra Smiljanic,Oleksandra Kaskun,Kimia Ghannad-Zadeh,Angela Celebre,Keren Isaev,A Sorana Morrissy,Jennifer Guan,Jiefei Tong,Jeffrey Chan,Taylor M Wilson,Sayf Al-Omaishi,David G Munoz,Peter B Dirks,Michael F Moran,Michael D Taylor,Jüri Reimand,Sunit Das

PloS one 7:e41105 PubMed22815931

2012

Amniotic mesenchymal stem cells enhance wound healing in diabetic NOD/SCID mice through high angiogenic and engraftment capabilities.

Applications

Unspecified application

Species

Human

Sung-Whan Kim,Hong-Zhe Zhang,Longzhe Guo,Jong-Min Kim,Moo Hyun Kim
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

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