Human EphA2 ELISA Kit
- Recombinant
- SimpleStep
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human EphA2 ELISA Kit (AB219051)
Example of human EphA2 standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human EphA2 ELISA Kit (AB219051)
Representative image of Human EphA2 ELISA Kit (ab219051)
Components shown from left to right :
- 10X Wash Buffer PT (ab206977)
- 10X Human EphA2 Capture Antibody
- 10X Human EphA2 Detector Antibody
- Human EphA2 Lyophilised Purified Protein (2 vials)
- 50X Cell Extraction Enhancer Solution (ab193971)
- Sample diluent NS
- TMB solution
- Stop Solution
- 5X Cell Extraction Buffer PTR (ab193970)
- Antibody diluent 5BI
SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.
Plate Seals - shown underneath bottles / vials.
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit
- sELISA
Supplier Data
Sandwich ELISA - Human EphA2 ELISA Kit (AB219051)
Interpolated concentrations of native EphA2 in human HepG2, HEK293T, and PC-3 cell extracts based on a 1,000 μg/mL extract load.
The concentrations of EphA2 were measured in duplicate, interpolated from the EphA2 standard curve, and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EphA2 concentration was determined to be 940 pg/mL in HepG2 cell extract, 1,076 pg/mL in HEK293T cell extract, and 17.4 ng/mL in PC-3 cell extract.
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제품 세부 정보
Human EphA2 ELISA Kit (ab219051) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EphA2 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EphA2 with 0.88 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Ephrin type-A Receptor 2, EPHA2, is a receptor tyrosine kinase encoded by the gene EPHA2. EphA2 is a 976-amino acid containing transmembrane glycoprotein, and is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. The extracellular portion of EphA2 contains the ephrin-A ligand binding site, and two fibronectin type-III repeats. EphA2 regulates cell adhesion and differentiation and is thought to be participate in mediating developmental events, especially in the nervous system. EphA2 has been shown to be highly expressed in tumor cell lines.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Eph receptor A2 participates in important cellular processes such as angiogenesis cell proliferation and migration. It often functions as part of larger signaling networks or complexes that include other Eph receptors and ephrin ligands. These interactions help regulate cell adhesion and detachment which are important for embryonic development and tissue organization. EphA2 studies often use fluorescent labeling strategies such as Alexa 588 to track its expression and activity in cells.
Pathways
Eph receptor A2 integrates into the PI3K/AKT and MAPK signaling pathways which are important for cell survival growth and differentiation. It interacts with proteins like Ras and ERK within these pathways to modulate their functions. EphA2 signaling influences cytoskeletal dynamics and morphogenesis making it an important component in maintaining tissue architecture. Research also indicates a connection between EphA2 and the APC protein highlighting its role in the Wnt signaling pathway.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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