AB274397

Human Erythropoietin ELISA Kit (EPO)

Name: Human Erythropoietin ELISA 키트 (EPO) (ab274397) | Abcam
Brand: Abcam Limited
SKU: AB274397
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Human Erythropoietin ELISA 키트 (EPO)

Recombinant
SimpleStep
각 태그의 뜻

(1 리뷰)

(2 제품이 사용된 논문 )

Human Erythropoietin ELISA Kit (EPO) is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify Human Erythropoietin (EPO) with a sensitivity of 1 mIU/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Need to design your own assay: we also offer the conjugation-ready antibody pair

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Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

sELISA

Supplier Data

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

Example of human Erythropoietin standard curve in Sample Diluent NS.

The Erythropoietin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

Interpolated concentrations of human Erythropoietin in HepG2 supernatant in 96-well vs. 384-well plates

Interpolated concentration of native Erythropoietin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

Example of human Erythropoietin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 23.4 mIU/mL with a range of 3.3 – 75.7 mIU/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

Interpolated concentrations of native Erythropoietin in human serum, plasma and HepG2 cell culture supernatant samples.

The concentrations of Erythropoietin were measured in duplicates, interpolated from the Erythropoietin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, Plasma (EDTA) 100%, plasma (heparin) 100% and Hep G2 supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Erythropoietin concentration was determined to be 16 mIU/mL in neat serum, 24 mIU/mL in neat plasma (citrate), 20 mIU/mL in neat plasma (EDTA), 20 mIU/mL in neat plasma (heparin) and 66 mIU/mL in neat Hep G2 supernatant.

sELISA

Lab

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1 mIU/mL

검출 범위

3.125 - 200 mIU/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human Erythropoietin ELISA Kit (EPO) ab274397 is a rapid single-wash 90-min ELISA kit to measure Human Erythropoietin (EPO) in cell culture supernatant, Serum and plasma. This SimpleStep ELISA® sensitivity is 1 mIU/mL.

How the assay works

Human Erythropoietin (EPO) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit is designed for the quantification of human Erythropoietin. The standard protein in this kit is mature full length human Erythropoietin. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin),and cell culture supernatant sample types. Spiked protein experiments were used to validate serum, plasma (citrate), plasma (EDTA), and plasma (heparin) sample types. Saliva, urine, milk, CSF, and cell and tissue extract samples have not been tested with this kit.

Human Erythropoietin ELISA Kit (EPO) ab274397 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

Save your precious samples

Our 384-well Human Erythropoietin ELISA Kit (EPO) only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- EPO Antibody Pair - BSA and Azide free ab253419

Related and recommended products

Human Erythropoietin ELISA Kit ab274397 is commonly used to study blood conditions such as anemia and polycythemia vera. Erythropoietin (EPO) plays a major role in the production of red blood cells.

Other ELISA kits to measure blood cell mechanisms and disorders are:

- Human Von Willebrand Factor ELISA kit ab223864
- Human ICAM1 ELISA kit (CD54) ab174445
- Human Leptin ELISA kit ab179884

Erythropoietin is the principal hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Erythropoietin binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. Erythropoietin is a secreted protein produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. Human erythropoietin is used for the treatment of anemia. Variations affecting the human EPO gene are associated with microvascular complications of diabetes 2. Human Erythropoietin has 83% amino acids sequence identity to rat Erythropoietin, and 80% identity to the mouse Erythropoietin.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "109 - 114 %", "average": "= 112" }, { "sample": "Serum", "range": "98 - 100 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "96 - 112 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "90 - 92 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "86 - 111 %", "average": "= 98" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass (PubMed : 28283061). Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3 (PubMed : 9774108).

See full target information EPO

대체 명칭 보기

Erythropoietin, EPO

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Bulletin of the World Health Organization 102:176-186 PubMed38420570

2024

Early iron supplementation in exclusively breastfed Gambian infants: a randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Mamadou Bah,Isabella Stelle,Hans Verhoef,Alasana Saidykhan,Sophie E Moore,Bubacarr Susso,Andrew M Prentice,Carla Cerami

American journal of physiology. Endocrinology and metabolism 324:E56-E61 PubMed36449571

2022

Ketone monoester ingestion increases postexercise serum erythropoietin concentrations in healthy men.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Evans,Jean-Philippe Walhin,Aaron Hengist,James A Betts,David J Dearlove,Javier T Gonzalez

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Erythropoietin ELISA Kit (EPO)

Human Erythropoietin ELISA 키트 (EPO)

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

Sandwich ELISA - Human Erythropoietin ELISA Kit (EPO) (AB274397)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise