AB322371

Human FABP-1 ELISA Kit- Intracellular

Recombinant
SimpleStep
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Human FABP-1 ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify Human FABP-1 with a sensitivity of 48.49 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

4 이미지

Sandwich ELISA - Human FABP-1 ELISA Kit- Intracellular (AB322371)

sELISA

Supplier Data

Sandwich ELISA - Human FABP-1 ELISA Kit- Intracellular (AB322371)

Example of human FABP-1 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human FABP-1 ELISA Kit- Intracellular (AB322371)

Interpolated concentrations of human FABP-1 in liver tissue extract in 96-well vs. 384-well plates. Interpolated concentration of native FABP-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 15 µg/mL liver tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human FABP-1 ELISA Kit- Intracellular (AB322371)

Interpolated concentration of native FABP-1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 cell extract 175 µg/mL, HeLa cell extract 25 µg/mL, and liver tissue extract 15 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human FABP-1 ELISA Kit- Intracellular (AB322371)

Example of human FABP-1 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate, Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 48.49 pg/mL

검출 범위

140.625 - 9000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human FABP-1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FABP-1 protein in Cell Lysate, Tissue extracts. Quantitate Human FABP-1 with 48.49 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.5" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "82 - 104 %", "average": "= 93" }, { "sample": "Tissue Extracts", "range": "100 - 120 %", "average": "= 110" } ]

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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab322371-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB322371-CMP11" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB322371-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322371-CMP06" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB322371-CMP09" }, { "size":"20 x 1 Vial", "name":"Human FABP-1 Lyophilized Recombinant Protein", "number":"AB322371-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human FABP-1 Detector Antibody", "number":"AB322371-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human FABP-1 Capture Antibody", "number":"AB322371-CMP01" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB322371-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322371-CMP10" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB322371-CMP04" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab322371-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322371-CMP11" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB322371-CMP09" }, { "size":"2 x 1 Vial", "name":"Human FABP-1 Lyophilized Recombinant Protein", "number":"AB322371-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human FABP-1 Detector Antibody", "number":"AB322371-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human FABP-1 Capture Antibody", "number":"AB322371-CMP01" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB322371-CMP08" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB322371-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB322371-CMP12" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322371-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB322371-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab322371-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322371-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FABP-1 Lyophilized Recombinant Protein", "number":"AB322371-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FABP-1 Detector Antibody", "number":"AB322371-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FABP-1 Capture Antibody", "number":"AB322371-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB322371-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322371-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB322371-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB322371-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322371-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322371-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB322371-CMP04", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.

See full target information GOT2

대체 명칭 보기

KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human FABP-1 ELISA Kit- Intracellular

Sandwich ELISA - Human FABP-1 ELISA Kit- Intracellular (AB322371)

Sandwich ELISA - Human FABP-1 ELISA Kit- Intracellular (AB322371)

Sandwich ELISA - Human FABP-1 ELISA Kit- Intracellular (AB322371)

Sandwich ELISA - Human FABP-1 ELISA Kit- Intracellular (AB322371)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Sample Prep & Detection Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise