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AB193700

Human FABP2 ELISA Kit

5

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(1 출판물)

Human FABP2 ELISA Kit is a Sandwich ELISA for the measurement of Human FABP2 in Human in Biofluids, Cell Culture Media samples.
1 이미지
Sandwich ELISA - Human FABP2 ELISA Kit (AB193700)
  • sELISA

Supplier Data

Sandwich ELISA - Human FABP2 ELISA Kit (AB193700)

Example Standard Curve

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture media, Serum

Reacts with

Human

분석 유형

Sandwich

Sensitivity

= 25 pg/mL

검출 범위

25 - 100000 pg/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

Abcam's FABP2 Human ELISA Kit (ab193700) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FABP2 in sera, plasma and cell culture supernatants.

This assay employs an antibody specific for human FABP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures FABP2 present in the samples. The wells are washed and biotinylated anti-human FABP2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of FABP2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

Recovery

[ { "sample": "Serum", "range": "120 - 136 %", "average": "= 129.8" }, { "sample": "Plasma", "range": "110 - 135 %", "average": "= 126.5" }, { "sample": "Cell culture media", "range": "103 - 123 %", "average": "= 112.4" } ]

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193700-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB193700-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193700-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193700-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human FABP2 Microplate (12 strips x 8 wells)", "number":"AB193700-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193700-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193700-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FABP2 Standards (lyophilized)", "number":"AB193700-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human FABP2 detection antibody", "number":"AB193700-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"1500X HRP-Streptavidin Concentrate", "number":"AB193700-CMP01", "productcode":"" } ] } } }

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C

제품 프로토콜

타겟 정보

FABPs are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
See full target information FABP2

대체 명칭 보기

FABPI, FABP2, Fatty acid-binding protein 2, Intestinal-type fatty acid-binding protein, I-FABP

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

BMC pediatrics 21:355 PubMed34418984

2021

Serum HMGB1 level is correlated with serum I-FABP level in neonatal patients with necrotizing enterocolitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruyahan Huo,Heng Liu,Jing Chen,Hong Sheng,Li Miao
제품이 사용된 논문 모두 보기
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