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AB300318

Human Factor B ELISA Kit

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Human Factor B ELISA Kit is a sandwich ELISA designed to quantify Human Factor B with a sensitivity of 18.18 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 53.125 - 3400 pg/mL
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ELISA - Human Factor B ELISA Kit (AB300318)
  • ELISA

Supplier Data

ELISA - Human Factor B ELISA Kit (AB300318)
Sandwich ELISA - Human Factor B ELISA Kit (AB300318)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)

Interpolated concentrations of human Factor B in serum, plasma (citrate), plasma (EDTA), and plasma (heparin).

Interpolated concentration of native Factor B was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 100,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)

Interpolated concentrations of human Factor B in HepG2 cell culture supernatant, saliva, and milk.

Interpolated concentration of native Factor B was measured in duplicate at different sample concentrations. Undiluted samples are as follows : HepG2 cell culture supernatant 1 : 8, saliva 1 : 16, and milk 1 : 800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)

Interpolated concentrations of Factor B in normal human serum donors.

Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor B concentration was determined to be 255 μg/mL with a range of 178 – 331 μg/mL.

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor B ELISA Kit (AB300318)

Example of human Factor B standard curve in Sample Diluent NS.

Example of human Factor B standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Milk, Saliva, Serum, Urine

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 18.18 pg/mL

검출 범위

53.125 - 3400 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Factor B ELISA Kit ab300318 is a rapid single-wash 90-min Sandwich ELISA to measure Human Factor B in cell culture supernatant, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 18.18 pg/mL.

How the assay works

Human Factor B SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Factor B ELISA Kit ab300318 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Factor B antibody [EPR24245-109] - BSA and Azide free (Capture) ab302722
- Anti-Factor B antibody [EPR24287-95] - BSA and Azide free (Detector) ab302723
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.72" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 100 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "103 - 110 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "92 - 106 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Serum", "range": "88 - 94 %", "average": "= 91" }, { "sample": "Saliva", "range": "82 - 88 %", "average": "= 85" }, { "sample": "Milk", "range": "90 - 100 %", "average": "= 94" }, { "sample": "Urine", "range": "82 - 83 %", "average": "= 82" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Factor B also known as B-factor is a protein that plays an important role in the immune system specifically in the complement pathway. It is sometimes referred to as factor B complement and its gene identifier is often referenced as KY21 NNL or factor B 1001. Factor B has a molecular mass of approximately 93 kDa. It is mainly expressed in the liver but can also be found in blood plasma in its inactive form as part of the innate immune response.
Biological function summary

Factor B is involved in the alternative complement pathway which serves as a part of the body's defense mechanism. It participates in the formation of the C3 convertase together with factor D and properdin initiating a cascade that can lead to the opsonization and elimination of pathogens. Factor B undergoes cleavage by factor D which generates the Ba and Bb fragments with Bb functioning as a serine protease within the C3 convertase complex.

Pathways

Factor B contributes to the complement system interacting notably in the alternative and lectin pathways. In these pathways Factor B associates with proteins like complement component C3. The alternative pathway is activated on microbial surfaces independent of antibodies. The involvement of Factor B in these pathways illustrates its role in pathogen recognition and further highlights its cooperation with complement proteins such as C3 and properdin.

Factor B has been associated with atypical hemolytic uremic syndrome (aHUS) a rare and serious disease affecting kidney function. Factor B involvement in aHUS links it to abnormal complement activation leading to a pathological condition. Additionally Factor B connects to age-related macular degeneration (AMD) a disorder where complement pathway genetic variations including those involving Factor B have shown associations. Understanding interactions with other complement components such as factor H helps to elucidate its role in these diseases.
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제품 프로토콜

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타겟 정보

Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases (PubMed : 6769474, PubMed : 9748277).. Complement factor B Bb. Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway (PubMed : 30643019, PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543). Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex (PubMed : 30643019, PubMed : 624565, PubMed : 6554279).. Complement factor B Ba. Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes.
See full target information CFB

대체 명칭 보기

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG
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websiteProtocolBooklet
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