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AB137975

Human Factor H ELISA Kit

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(5 제품이 사용된 논문 )

Human Factor H ELISA Kit is a sandwich ELISA designed to quantify Human Factor H with a sensitivity of 0.14 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 1.125 - 9 ng/mL
5 이미지
ELISA - Human Factor H ELISA Kit (AB137975)
  • ELISA

Lab

ELISA - Human Factor H ELISA Kit (AB137975)

Standard curve with background signal subtracted (duplicates; +/- SD).

Sandwich ELISA - Human Factor H ELISA Kit (AB137975)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor H ELISA Kit (AB137975)

Representative Standard Curve using ab137975

Sandwich ELISA - Human Factor H ELISA Kit (AB137975)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor H ELISA Kit (AB137975)

Representative Standard Curve using ab137975

ELISA - Human Factor H ELISA Kit (AB137975)
  • ELISA

Lab

ELISA - Human Factor H ELISA Kit (AB137975)

Factor H measured in culture supernatants (tested at dilution range 1/1-1/30; duplicates +/- SD).

ELISA - Human Factor H ELISA Kit (AB137975)
  • ELISA

Lab

ELISA - Human Factor H ELISA Kit (AB137975)

Factor H measured in biological fluids (duplicates +/- SD). Human serum and plasma were tested at dilution range of 1/50000-1/1500000. Other bilogical fluids were tested at 1/1-1/500 and gave measurable values (milk : 400, saliva : 200, CSF : 2000 and urine : 40 ng per mL).

주요 정보

검출 방식

Colorimetric

샘플 타입

Urine, Saliva, Milk, Plasma, Serum, Cerebral Spinal Fluid

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.16 ng/mL

검출 범위

0.281 - 18 ng/mL

분석 소요 시간

4h

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Factor H ELISA Kit ab137975 is a sandwich ELISA to measure Human Factor H in serum, plasma, cell culture supernatant, cerebral spinal fluid (CSF), saliva, urine, milk with a sensitivity of 0.14 ng/ml.

How the assay works

A Factor H specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor H specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor H captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Factor H ELISA Kit ab137975 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Factor H also known as complement factor H is an important regulatory protein in the complement system. It has a molecular mass of approximately 155 kDa. This protein is mainly expressed in the liver but it can also be found in low levels in other tissues. Factor H serves as a control element for complement activation particularly affecting the alternative pathway. It binds to C3b a central component of the complement system and accelerates the decay of C3 convertase as well as promotes the proteolytic inactivation of C3b by factor I.
Biological function summary

Factor H limits the activity of the complement system to prevent damage to host tissues. The protein exists in the plasma in a soluble form. It functions by recognizing host cell surfaces via specific markers avoiding inappropriate activation. Factor H belongs to a group of proteins which include other regulators of complement activation. These proteins maintain the balance between effective immune defense and protection of host tissue from excessive immune responses.

Pathways

Factor H is a part of the alternative complement pathway. This pathway is important for innate immune response involving proteins like factor P (properdin) which stabilizes C3 convertase. Factor H modulates these interactions to prevent unwarranted complement activity on host cells. Another related pathway is the classic complement pathway although factor H's involvement here is less direct since it primarily regulates the alternative pathway.

Factor H associations include atypical hemolytic uremic syndrome and age-related macular degeneration. Factor H deficiency or dysfunction can lead to uncontrolled complement activation resulting in kidney damage in atypical hemolytic uremic syndrome where it is also related to factor I. Additionally in age-related macular degeneration variants in the factor H gene are linked to increased susceptibility further highlighting the protein's importance in regulating immune responses.
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제품 프로토콜

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타겟 정보

Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed : 21285368, PubMed : 21317894, PubMed : 25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed : 19503104, PubMed : 21317894, PubMed : 26700768). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed : 18252712, PubMed : 23332154, PubMed : 28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed : 20008295, PubMed : 9558116).. (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.
See full target information CFH

대체 명칭 보기

HF, HF1, HF2, CFH, Complement factor H, H factor 1
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

ERJ open research 9: PubMed37377659

2023

Factor H preserves alternative complement function during ARDS, linked to improved survival.

Applications

Unspecified application

Species

Unspecified reactive species

William Bain,Mohammadreza Tabary,Sara R Moore,Xiaojing An,Georgios D Kitsios,Bryan J McVerry,Prabir Ray,Anuradha Ray,Rama K Mallampalli,Viviana P Ferreira,Janet S Lee,S Mehdi Nouraie

European cells & materials 42:1-19 PubMed34235715

2021

Mesenchymal stem cell secretome decreases the inflammatory response in annulus fibrosus organ cultures.

Applications

Unspecified application

Species

Unspecified reactive species

C Neidlinger-Wilke,A Ekkerlein,R M Goncalves,J R Ferreira,A Ignatius,H J Wilke,G Q Teixeira

Frontiers in immunology 12:660194 PubMed33868311

2021

A Family Affair: Addressing the Challenges of Factor H and the Related Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Poppelaars,Elena Goicoechea de Jorge,Ilse Jongerius,Antje J Baeumner,Mark-Steven Steiner,Mihály Józsi,Erik J M Toonen,Diana Pauly

Journal of immunology (Baltimore, Md. : 1950) 202:1210-1218 PubMed30642982

2019

Cadmium Induces Glomerular Endothelial Cell-Specific Expression of Complement Factor H via the -1635 AP-1 Binding Site.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaocui Chen,Liqun Li,Fuhong Liu,Josephine Hoh,Carolyn M Kapron,Ju Liu

The Journal of clinical investigation 127:199-214 PubMed27918307

2016

VEGF regulates local inhibitory complement proteins in the eye and kidney.

Applications

ELISA

Species

Human

Lindsay S Keir,Rachel Firth,Lyndsey Aponik,Daniel Feitelberg,Susumu Sakimoto,Edith Aguilar,Gavin I Welsh,Anna Richards,Yoshihiko Usui,Simon C Satchell,Valeryia Kuzmuk,Richard J Coward,Jonathan Goult,Katherine R Bull,Ruchi Sharma,Kapil Bharti,Peter D Westenskow,Iacovos P Michael,Moin A Saleem,Martin Friedlander
제품이 사용된 논문 모두 보기
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