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AB168546

Human Factor IX ELISA Kit (with plasma controls)

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Human Factor IX ELISA Kit (with plasma controls) is a Sandwich ELISA for the measurement of Human Factor IX (with plasma controls) in Human in Biofluids samples.
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Sandwich ELISA - Human Factor IX ELISA Kit (with plasma controls) (AB168546)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX ELISA Kit (with plasma controls) (AB168546)

Human Factor IX ELISA Kit (with plasma controls).

Representative Standard Curve using ab168546

주요 정보

검출 방식

Colorimetric

샘플 타입

Cerebral Spinal Fluid, Plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.33 ng/mL

검출 범위

1.56 - 100 ng/mL

분석 소요 시간

4h

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's Factor IX Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor IX levels in CSF, serum and plasma.

A Factor IX specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor IX specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor IX captured in plate.

Get higher sensitivity in only 90 minutes with Human Factor IX ELISA Kit (ab188393) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab168546-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Factor IX Microplate (12 x 8 well strips)", "number":"AB168546-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Factor IX Standard", "number":"AB168546-CMP04", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB168546-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB168546-CMP01", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB168546-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB168546-CMP10", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB168546-CMP06", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB168546-CMP11", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Factor IX Antibody", "number":"AB168546-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive control (Reference Plasma Control)", "number":"AB168546-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Low control (Depleted Human Plasma)", "number":"AB168546-CMP08", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Factor IX also known as Christmas factor or PTC is an important clotting protein in the coagulation cascade. It is a 415 amino acid protein with a mass of approximately 55 kDa. Factor IX is mainly expressed in the liver and then released into the bloodstream where it plays a significant role in blood clotting processes. The recombinant version of this protein referred to as factor IX recombinant is used therapeutically especially in patients who require coagulation support due to factor deficiencies.
Biological function summary

The function of factor IX involves its role in the amplification phase of the coagulation cascade. It participates as part of the intrinsic tenase complex which forms on negatively charged phospholipids in the presence of calcium ions. This complex significantly enhances the conversion of factor X to its active form factor Xa an important step in the clotting process. The activity of factor IX can be assessed using assays such as the factor IX activity assay and ELISA allowing for precise measurement of its function and quantity.

Pathways

Factor IX is integral within the intrinsic pathway of blood coagulation. It interacts with proteins such as factor VIII and factor X. When activated factor IXa forms a complex with the cofactor factor VIIIa on membrane surfaces intensifying the transformation of factor X to factor Xa which then catalyzes the conversion of prothrombin to thrombin. This series of reactions leads to fibrin formation important for stable clot production. Factor IXa also relates to the pathways influencing cellular signaling and hemostasis.

Factor IX deficiencies cause hemophilia B a genetic disorder that leads to improper blood clotting. This disorder is mainly characterized by spontaneous bleeding or severe bleeding after injury. Patients with hemophilia B usually have mutations in the factor IX gene on the X chromosome affecting the protein's function. Successful treatment often involves replacement therapy using factor IX concentrates or the recombinant form. The interaction with proteases such as factor VIIa highlights its importance in therapeutic strategies for bleeding disorders.
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제품 프로토콜

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타겟 정보

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa (PubMed : 8295821, PubMed : 2592373, PubMed : 20121197, PubMed : 20121198, PubMed : 1730085, PubMed : 19846852, PubMed : 39880037).
See full target information Coagulation factor IX

대체 명칭 보기

Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9
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websiteProtocolBooklet
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Product promise

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