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AB300307

Human Factor IX/PTC ELISA Kit

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Human Factor IX/PTC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Factor IX/PTC with a sensitivity of 15.9 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Interpolated concentrations of Factor IX/PTC in normal human serum donors

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Factor IX/PTC concentration was determined to be 4,867.18 ng/mL with a range of 1,062.16 – 8,981.25 ng/mL.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Interpolated concentrations of human Factor IX/PTC in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)

Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 0.15%, plasma (citrate) 0.075%, plasma (EDTA) 0.075%, and plasma (heparin) 0.15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Example of human Factor IX/PTC standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Example of human Factor IX/PTC standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Example of Human Factor IX/PTC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Interpolated concentrations of human Factor IX/PTC in serum in 96-well vs. 384-well plates

Interpolated concentration of native Factor IX/PTC was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 400 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Human Factor IX/PTC serum and plasma comparison

Serum and plasma comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300307).

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Supplier Data

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

Standard curve comparison between the original Human Factor IX/PTC SimpleStep ELISA (ab188393) and current Human Factor IX/PTC SimpleStep ELISA (ab300307).

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)
  • sELISA

Lab

Sandwich ELISA - Human Factor IX/PTC ELISA Kit (AB300307)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

주요 정보

검출 방식

Colorimetric

샘플 타입

Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 15.884 pg/mL

검출 범위

97.656 - 6250 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Factor IX/PTC ELISA Kit ab300307 is a rapid single-wash 90-min ELISA kit to measure Human Factor IX/PTC in serum and plasma. This SimpleStep ELISA® sensitivity is 15.884 pg/mL.

How the assay works
Human Factor IX/PTC SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit is designed for the quantification of human Factor IX/PTC. The standard protein in this kit is full length human Factor IX/PTC. Native signal was detected in serum, plasma (heparin), plasma (EDTA), and plasma (citrate). Cell culture supernatant, saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit.

Human Factor IX/PTC ELISA Kit ab300307 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

Save your precious samples
Our 384-well Human Factor IX/PTC ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Factor IX (F9) Antibody Pair - BSA and Azide free ab253721
- Anti-Factor IX/PTC antibody [EPR22846-57] - BSA and Azide free (Capture) ab281281
- Anti-Factor IX/PTC antibody [EPR22846-233] - BSA and Azide free (Detector) ab281131

Related and recommended products
Human Factor IX/PTC ELISA Kit ab300307 is commonly used to study cardiovascular diseases and clotting disorders.

Other ELISA kits to measure inflammation and coagulation include:
- Human Factor X ELISA Kit ab108832
- Human Lipocalin-2 ELISA Kit ab215541

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.4" } ]
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Recovery

[ { "sample": "Citrate plasma", "range": "111 - 119 %", "average": "= 116" }, { "sample": "EDTA Plasma", "range": "95 - 115 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "103 - 118 %", "average": "= 112" }, { "sample": "Serum", "range": "92 - 113 %", "average": "= 105" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Factor IX also known as Christmas factor or PTC is an important clotting protein in the coagulation cascade. It is a 415 amino acid protein with a mass of approximately 55 kDa. Factor IX is mainly expressed in the liver and then released into the bloodstream where it plays a significant role in blood clotting processes. The recombinant version of this protein referred to as factor IX recombinant is used therapeutically especially in patients who require coagulation support due to factor deficiencies.
Biological function summary

The function of factor IX involves its role in the amplification phase of the coagulation cascade. It participates as part of the intrinsic tenase complex which forms on negatively charged phospholipids in the presence of calcium ions. This complex significantly enhances the conversion of factor X to its active form factor Xa an important step in the clotting process. The activity of factor IX can be assessed using assays such as the factor IX activity assay and ELISA allowing for precise measurement of its function and quantity.

Pathways

Factor IX is integral within the intrinsic pathway of blood coagulation. It interacts with proteins such as factor VIII and factor X. When activated factor IXa forms a complex with the cofactor factor VIIIa on membrane surfaces intensifying the transformation of factor X to factor Xa which then catalyzes the conversion of prothrombin to thrombin. This series of reactions leads to fibrin formation important for stable clot production. Factor IXa also relates to the pathways influencing cellular signaling and hemostasis.

Factor IX deficiencies cause hemophilia B a genetic disorder that leads to improper blood clotting. This disorder is mainly characterized by spontaneous bleeding or severe bleeding after injury. Patients with hemophilia B usually have mutations in the factor IX gene on the X chromosome affecting the protein's function. Successful treatment often involves replacement therapy using factor IX concentrates or the recombinant form. The interaction with proteases such as factor VIIa highlights its importance in therapeutic strategies for bleeding disorders.
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제품 프로토콜

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타겟 정보

Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa (PubMed : 8295821, PubMed : 2592373, PubMed : 20121197, PubMed : 20121198, PubMed : 1730085, PubMed : 19846852, PubMed : 39880037).
See full target information Coagulation factor IX

대체 명칭 보기

Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTC, F9
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websiteProtocolBooklet
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