Human FAS ELISA Kit
- Recombinant
- SimpleStep
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human FAS ELISA Kit (AB183360)
Interpolated concentrations of native FAS in human serum and plasma samples.
The concentrations of FAS were measured in duplicates, interpolated from the FAS standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate, EDTA, heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 1,393 pg/mL in neat serum, 1,231 pg/mL in neat plasma (citrate) and 1,226 pg/mL in neat plasma (EDTA) and 1,237pg/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human FAS ELISA Kit (AB183360)
Interpolated concentrations of native FAS in human PBMC cell culture supernatant derived from cells seeded at 107 cells/mL and cultured in the presence or absence of 1.5% PHA for 36 hours.
The concentrations of FAS were measured in duplicate and interpolated from the FAS standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 157 pg/mL in neat stimulated PBMC cell culture supernatant and undetectable in unstimulated PBMC cell culture supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human FAS ELISA Kit (AB183360)
Human FAS standard curve comparison.
Standard Curve comparison between the original Human FAS SimpleStep ELISA kit and current Human FAS SimpleStep ELISA kit.
- sELISA
Supplier Data
Sandwich ELISA - Human FAS ELISA Kit (AB183360)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 1,513 pg/mL with a range of 1,050 – 2,009 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human FAS ELISA Kit (AB183360)
Example of human FAS standard curve in Sample Diluent NS.
The FAS standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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As of October 15, 2019, Human FAS ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.Human FAS ELISA kit (ab183360) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FAS protein in human cell culture supernatant, serum, and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human FAS with 2 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
FAS is a plasma membrane receptor for FASLG ligand (TNFSF6/FASLG). Upon ligand binding, the FASLG Receptor is activated and the adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) activation mediating apoptosis. FASLG-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 of the FASLG Receptor block apoptosis as they sequester the Fas ligand.
This kit uses a recombinant protein fragment corresponding to the extracellular portion (amino acid 17-172) of the full length Human Fas Receptor protein as standard sample.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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