AB100515

Human Fas Ligand ELISA Kit (APTL)

Name: Human Fas Ligand ELISA 키트 (APTL) (ab100515) | Abcam
Brand: Abcam Limited
SKU: AB100515
Rating: 4 (2 reviews)

Human Fas Ligand ELISA 키트 (APTL)

(2 리뷰들)

(4 제품이 사용된 논문 )

Human Fas Ligand ELISA Kit (APTL) is a Sandwich ELISA for the measurement of Human Fas Ligand (APTL) in Human in Biofluids, Cell Culture Media samples.

대체 명칭 보기

CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL

2 이미지

Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (AB100515)

sELISA

Supplier Data

Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (AB100515)

Representative Standard Curve using ab100515.

sELISA

Supplier Data

Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (AB100515)

Representative Standard Curve using ab100515.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 2 pg/mL

검출 범위

1.37 - 1000 pg/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Abcam's Fas Ligand (APTL) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas Ligand in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human Fas Ligand coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas Ligand present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Fas Ligand antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas Ligand bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "70 - 90 %", "average": "= 76.99" }, { "sample": "Serum", "range": "70 - 87 %", "average": "= 76.36" }, { "sample": "Plasma", "range": "67 - 86 %", "average": "= 73.42" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100515-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100515-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100515-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100515-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fas Ligand Microplate (12 x 8 wells)", "number":"AB100515-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100515-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100515-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Fas Ligand Standard (lyophilized)", "number":"AB100515-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Fas Ligand", "number":"AB100515-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100515-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

-20°C

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제품 프로토콜

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타겟 정보

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed : 26334989, PubMed : 9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed : 7528780, PubMed : 9228058, PubMed : 9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis also has a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed : 27806260).. Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity). Can induce apoptosis but does not appear to be essential for this process (PubMed : 27806260).. FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.

See full target information FASLG

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Microbiology spectrum 10:e0135722 PubMed35938810

2022

Mixed T Helper1/T Helper2/T Cytotoxic Profile in Subjects with Chronic Chagas Disease with Hypersensitivity Reactions to Benznidazole.

Applications

Unspecified application

Species

Unspecified reactive species

Melisa D Castro Eiro,María A Natale,María G Alvarez,Araceli Castro,Débora Seigelshifer,Rodolfo Viotti,Marisa Fernández,Luis Mazzuoccolo,Bruno Lococo,Graciela L Bertocchi,Gonzalo Cesar,María C Albareda,María J Elias,María B Caputo,Eduardo Gaddi,Jeanette Balbaryski,Carlos A Vigliano,Susana A Laucella

Frontiers in physiology 12:708824 PubMed34366896

2021

Assessment of Placental Extracellular Vesicles-Associated Fas Ligand and TNF-Related Apoptosis-Inducing Ligand in Pregnancies Complicated by Early and Late Onset Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Ayala-Ramírez,Catalina Machuca-Acevedo,Tatiana Gámez,Sandra Quijano,Alfonso Barreto,Jaime L Silva,Mercedes Olaya-C,Reggie García-Robles

Journal of extracellular vesicles 10:e12022 PubMed33708355

2020

Microglial derived extracellular vesicles activate autophagy and mediate multi-target signaling to maintain cellular homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Bram Van den Broek,Isabel Pintelon,Ibrahim Hamad,Sofie Kessels,Mansour Haidar,Niels Hellings,Jerome J A Hendriks,Markus Kleinewietfeld,Bert Brône,Vincent Timmerman,Jean-Pierre Timmermans,Veerle Somers,Luc Michiels,Joy Irobi

Frontiers in pediatrics 6:139 PubMed29977885

2018

Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Zhang,Xinlun Dai,Ji Qi,Yu Ao,Chunfeng Yang,Yumei Li

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Fas Ligand ELISA Kit (APTL)

Human Fas Ligand ELISA 키트 (APTL)

Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (AB100515)

Sandwich ELISA - Human Fas Ligand ELISA Kit (APTL) (AB100515)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise