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AB108698

Human Ferritin ELISA Kit

5

(1 리뷰)

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(11 제품이 사용된 논문 )

Human Ferritin ELISA Kit is a sandwich ELISA designed to quantify Human Ferritin with a sensitivity of 0.53 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Cited in over 10 publications

대체 명칭 보기

Ferritin light chain, Ferritin L subunit, FTL

1 이미지
Sandwich ELISA - Human Ferritin ELISA Kit (AB108698)
  • sELISA

Unknown

Sandwich ELISA - Human Ferritin ELISA Kit (AB108698)

Representative Standard Curve using ab108698

주요 정보

검출 방식

Colorimetric

샘플 타입

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.53 ng/mL

분석 플랫폼

Microplate

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보완 제품

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AB108837

Human Ferritin ELISA Kit

Sandwich ELISA - Human Ferritin ELISA Kit (AB108837)

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Human FGL1 ELISA Kit

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ELISA - Human FGL1 ELISA Kit (AB284622)

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AB282302

Human Polymeric immunoglobulin receptor ELISA Kit (PIGR)

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ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)

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Assay Kits

AB222511

Mouse C Reactive Protein ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse C Reactive Protein ELISA Kit (AB222511)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Ferritin ELISA Kit ab108698 is a sandwich ELISA to measure Human Ferritin in serum, citrate plasma, heparin plasma with a sensitivity of 0.53 ng/ml.

How the assay works

A 96-well plate has been precoated with anti-Ferritin IgG antibodies. Samples, standards and the Ferritin-HRP conjugate are added to the wells, where Ferritin in the sample and standards binds to the precoated antibody and added Ferritin-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Ferritin in the sample and the intensity is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Ferritin ELISA Kit ab108698 protocol summary

1. Add samples, standards and controls to wells used.
2. Add prepared labeled HRPConjugate to each well. Incubate at room temperature.
3. After washing, add TMB substrate solution to each well. Incubate at room temperature.
4. Add Stop Solution to each well. Read immediately.
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Precision

[ { "reproducibilityType": "Intra", "sample": "Control", "replicates": 48, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 5.4" }, { "reproducibilityType": "Inter", "sample": "Control", "replicates": 48, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 6.1" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108698-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Anti-Ferritin IgG Coated Microplate (12 x 8 wells)", "number":"AB108698-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108698-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108698-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108698-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"10X Washing Solution", "number":"AB108698-CMP13", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108698-CMP14", "productcode":"" }, { "size":"1 x 1 mL", "name":"Ferritin Standard 2 - 20 ng/mL", "number":"AB108698-CMP08", "productcode":"" }, { "size":"1 x 3 mL", "name":"Ferritin Standard 0 - 0 ng/mL", "number":"AB108698-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"Ferritin Standard 3 - 100 ng/mL", "number":"AB108698-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"Ferritin Standard 5 - 800 ng/mL", "number":"AB108698-CMP15", "productcode":"" }, { "size":"1 x 1 mL", "name":"Ferritin Standard 1 - 5 ng/mL", "number":"AB108698-CMP07", "productcode":"" }, { "size":"1 x 1 mL", "name":"Ferritin Standard 4 - 400 ng/mL", "number":"AB108698-CMP10", "productcode":"" }, { "size":"1 x 21 mL", "name":"Anti-Ferritin-HRP conjugate", "number":"AB108698-CMP01", "productcode":"" }, { "size":"1 x 1 mL", "name":"Ferritin Control", "number":"AB108698-CMP05", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed : 24695223).
See full target information FTL
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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal for equity in health 23:200 PubMed39369256

2024

Biological and contextual determinants of early development in marginalized Roma communities: A research protocol of the RomaREACH study.

Applications

Unspecified application

Species

Unspecified reactive species

Shoshana Chovan,Daniela Fiľakovská Bobáková,Andrea Madarasová Gecková,Beáta Hubková,Gabriela Štrkolcová,Sijmen A Reijneveld,Marlou L A de Kroon

Cureus 16:e60422 PubMed38883134

2024

Physiological Role of Serum Growth Differentiation Factor-15 (GDF-15) Level and Iron Metabolism in Community-Dwelling Older Adults.

Applications

Unspecified application

Species

Unspecified reactive species

Taira Fukuda,Hiroko Yazawa,Riichi Nishikawa,Seiko Tokoi,Ryo Kayashima,Kaori Kono,Masashi Sakuma,Shichiro Abe,Shigeru Toyoda,Toshiaki Nakajima

Animals : an open access journal from MDPI 13: PubMed36899749

2023

Immunological and Oxidative Biomarkers in Bovine Serum from Healthy, Clinical, and Sub-Clinical Mastitis Caused by and Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Asmaa Sadat,Alshimaa M M Farag,Driss Elhanafi,Amal Awad,Ehab Kotb Elmahallawy,Noorah Alsowayeh,Manal F El-Khadragy,Gehad E Elshopakey

Drug testing and analysis 15:444-448 PubMed36354188

2022

The effects of iron injection on blood doping biomarkers in dried blood spots.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Loria,Estelle Maret,Céline Schobinger,Tiia Kuuranne,Silke Grabherr,Nicolas Leuenberger

Journal of clinical medicine 10: PubMed34209847

2021

Impaired Iron Homeostasis and Haematopoiesis Impacts Inflammation in the Ageing Process in Down Syndrome Dementia.

Applications

Unspecified application

Species

Unspecified reactive species

Ruma Raha-Chowdhury,Animesh Alexander Raha,James Henderson,Seyedeh Deniz Ghaffari,Monika Grigorova,Jessica Beresford-Webb,Kieren Allinson,Subhojit Chakraborty,Anthony Holland,Shahid H Zaman

Frontiers in aging neuroscience 13:653591 PubMed33994996

2021

Hepcidin Increases Cytokines in Alzheimer's Disease and Down's Syndrome Dementia: Implication of Impaired Iron Homeostasis in Neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Animesh Alexander Raha,Seyedeh Deniz Ghaffari,James Henderson,Subhojit Chakraborty,Kieren Allinson,Robert P Friedland,Anthony Holland,Shahid H Zaman,Elizabeta B Mukaetova-Ladinska,Ruma Raha-Chowdhury

Transfusion 60:2982-2990 PubMed32945551

2020

Hemochromatosis alters the sensitivity of red blood cells to mechanical stress.

Applications

Unspecified application

Species

Unspecified reactive species

Kieran J Richardson,Antony P McNamee,Michael J Simmonds

Transfusion 60:3001-3009 PubMed32939772

2020

Mechanical sensitivity of red blood cells improves in individuals with hemochromatosis following venesection therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Kieran J Richardson,Antony P McNamee,Michael J Simmonds

PloS one 15:e0227965 PubMed31971986

2020

Prevalence and foetomaternal effects of iron deficiency anaemia among pregnant women in Lagos, Nigeria.

Applications

Unspecified application

Species

Unspecified reactive species

Adegbenga Adetona Ajepe,Kehinde Sharafadeen Okunade,Adebayo Isaiah Sekumade,Ebunoluwa Seun Daramola,Mary Olufunmilayo Beke,Olaolopin Ijasan,Olusola Festus Olowoselu,Bukola Bosede Afolabi

Scientific reports 9:2918 PubMed30814647

2019

A ferroptosis-based panel of prognostic biomarkers for Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

David Devos,Caroline Moreau,Maeva Kyheng,Guillaume Garçon,Anne Sophie Rolland,Hélène Blasco,Patrick Gelé,T Timothée Lenglet,C Veyrat-Durebex,Philippe Corcia,Mary Dutheil,Peter Bede,Andreas Jeromin,Patrick Oeckl,Markus Otto,Vincent Meininger,Véronique Danel-Brunaud,Jean-Christophe Devedjian,James A Duce,Pierre François Pradat
제품이 사용된 논문 모두 보기
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