AB267652

Human FGF23 ELISA Kit

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Human FGF23 ELISA Kit is a Sandwich ELISA for the measurement of Human FGF23 in Human in Biofluids, Cell Culture Media samples.

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Sandwich ELISA - Human FGF23 ELISA Kit (AB267652)

sELISA

Supplier Data

Sandwich ELISA - Human FGF23 ELISA Kit (AB267652)

Human FGF23 ELISA kit (ab267652) Standard curve.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture media, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.3 ng/mL

검출 범위

0.307 - 75 ng/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human FGF23 ELISA Kit is designed for the quantitative determination of FGF23 in cell culture supernatants, plasma and serum samples.

ΔNote: Human FGF-23 concentration is low in normal serum/plasma, and may not be detectable in this assay.

This assay employs an antibody specific for human FGF23 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF23 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human FGF23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF23 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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Recovery

[ { "sample": "Serum", "range": "122 - 145 %", "average": "= 135" }, { "sample": "Plasma", "range": "138 - 147 %", "average": "= 144" }, { "sample": "Cell culture media", "range": "133 - 149 %", "average": "= 143" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267652-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB267652-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267652-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267652-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267652-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human FGF23 coated Microplate (12 x 8 wells)", "number":"AB267652-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267652-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"350X HRP-Streptavidin Concentrate", "number":"AB267652-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FGF23 Standard (Lyophilized)", "number":"AB267652-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human FGF23 Detection Antibody", "number":"AB267652-CMP08", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Fibroblast Growth Factor 23 also known as FGF 23 is a hormone-like protein that plays a role in phosphate and vitamin D metabolism. It is a member of the fibroblast growth factor family and consists of about 251 amino acids with a mass of approximately 32 kDa. FGF 23 is predominantly expressed in bone cells especially osteocytes but it also appears in other tissues such as the thymus and brain. An important tool for quantitative measurement of FGF 23 levels is the FGF 23 ELISA kit which assists in various research and clinical settings.

Biological function summary

FGF 23 plays an important role in regulating phosphate balance in the body. It impacts the function of kidneys by decreasing the reabsorption of phosphate and suppressing the formation of active vitamin D thereby affecting calcium regulation as well. FGF 23 does not operate alone but interacts with co-receptors such as Klotho to activate its receptor complex. The protein functions as part of a larger system that fine-tunes the balance of minerals important for maintaining bone health and metabolic functions.

Pathways

FGF 23 is part of the bone-mineral metabolism axis and is integral to the phosphate regulation pathway. It works closely with proteins like Klotho and FGFR1 and their interaction ensures proper signaling required for maintaining serum phosphate levels. FGF 23 controls vitamin D metabolism by regulating the expression of 1-alpha-hydroxylase and 24-hydroxylase influencing the activity of the related vitamin D endocrine system. Its actions help stabilize phosphate and vitamin D status which are both essential for skeletal and mineral homeostasis.

FGF 23 has a significant connection to conditions such as Hypophosphatemic Rickets and Chronic Kidney Disease (CKD). In these cases FGF 23 levels may become dysregulated leading to abnormal phosphate metabolism and bone mineralization defects. In Hypophosphatemic Rickets altered expression of FGF 23 causes excessive phosphate wasting whereas in CKD increased FGF 23 levels are associated with cardiovascular disease risk and deteriorating kidney function. Klotho as a coreceptor for FGF 23 also influences these pathological conditions highlighting its contribution to phosphate-related disorder mechanisms.

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제품 프로토콜

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타겟 정보

Regulator of phosphate homeostasis (PubMed : 11062477). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (PubMed : 11409890). Up-regulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism (PubMed : 15040831). Negatively regulates osteoblast differentiation and matrix mineralization (PubMed : 18282132).

See full target information FGF23

대체 명칭 보기

HYPF, UNQ3027/PRO9828, FGF23, Fibroblast growth factor 23, FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Clinical interventions in aging 19:715-725 PubMed38716143

2024

Predictive Value of Serum microRNA-29b-3p in Recurrence of Atrial Fibrillation After Radiofrequency Catheter Ablation.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei Zhan,Chengfei Peng,Yuxin Liu,Zhanhua Bi,Guoxiu Lu,Shanhu Hao,Yanan Tong,Guoxu Zhang

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Human FGF23 ELISA Kit

Sandwich ELISA - Human FGF23 ELISA Kit (AB267652)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise