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AB108848

Human Fibronectin ELISA Kit

5

(2 리뷰들)

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(4 제품이 사용된 논문 )

Human Fibronectin ELISA Kit is a Competitive ELISA for the measurement of Human Fibronectin in Human in Biofluids samples.
2 이미지
Competitive ELISA - Human Fibronectin ELISA Kit (AB108848)
  • cELISA

Lab

Competitive ELISA - Human Fibronectin ELISA Kit (AB108848)

Fibronectin measured in various samples showing quantity (microgram) per mL of tested sample

Competitive ELISA - Human Fibronectin ELISA Kit (AB108848)
  • cELISA

Unknown

Competitive ELISA - Human Fibronectin ELISA Kit (AB108848)

Representative Standard Curve using ab108848.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Serum

Reacts with

Human

분석 유형

Competitive

Sensitivity

= 0.61 µg/mL

검출 범위

0.781 - 50 µg/mL

분석 소요 시간

3h

분석 플랫폼

Microplate

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보완 제품

Assay Kits

AB210966

Human Pro-Collagen I alpha 1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

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AB108804

Human Apolipoprotein AI ELISA Kit (APOA1)

Competitive ELISA - Human Apolipoprotein AI ELISA Kit (APOA1) (AB108804)

  • 4
  • 3
Assay Kits

AB187394

Human TIMP1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human TIMP1 ELISA Kit (AB187394)

  • 0
  • 0
Assay Kits

AB269371

Human MMP3 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MMP3 ELISA Kit (AB269371)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's Fibronectin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Fibronectin levels in plasma and serum.

A Fibronectin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Fibronectin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibronectin captured in plate.

Get results in 90 minutes with Human Fibronectin ELISA Kit (ab181419) from our SimpleStep ELISA® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108848-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Fibronectin Microplate (12 x 8 well strips)", "number":"AB108848-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB108848-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB108848-CMP01", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB108848-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB108848-CMP08", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB108848-CMP07", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB108848-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Fibronectin Standard", "number":"AB108848-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"1X Biotinylated Human Fibronectin (Lyophilized)", "number":"AB108848-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
Multi
보관 정보
Please refer to protocols
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제품 프로토콜

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타겟 정보

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.
See full target information FN1

대체 명칭 보기

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Endocrine 83:227-241 PubMed37653215

2023

Effects and plasma proteomic analysis of GLP-1RA versus CPA/EE, in combination with metformin, on overweight PCOS women: a randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyu Liao,Xing Li,Hao Zhang,Ling Zhou,Liu Shi,Weixin Li,Rufei Shen,Guiliang Peng,Huan Zhao,Jiaqing Shao,Xiujie Wang,Zheng Sun,Hongting Zheng,Min Long

Placenta 139:112-119 PubMed37356366

2023

Early pregnancy biomarker discovery study for spontaneous preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Rik H J Beernink,Joost H N Schuitemaker,Eva F Zwertbroek,Sicco A Scherjon,Thomas I F H Cremers

Anticancer research 36:665-71 PubMed26851022

2016

L-Carnosine Prevents the Pro-cancerogenic Activity of Senescent Peritoneal Mesothelium Towards Ovarian Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Justyna Mikuła-Pietrasik,Krzysztof Książek

Journal of photochemistry and photobiology. B, Bio 144:94-103 PubMed25732262

2015

A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Di Cerbo,Carmen Laurino,Beniamino Palmieri,Tommaso Iannitti
제품이 사용된 논문 모두 보기
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Product promise

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