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AB213779

Human Ficolin-3 ELISA Kit

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Human Ficolin-3 ELISA Kit is a Sandwich ELISA for the measurement of Human Ficolin-3 in Human in Cell Culture Media, Biofluids samples.

대체 명칭 보기

FCNH, HAKA1, FCN3, Ficolin-3, Collagen/fibrinogen domain-containing lectin 3 p35, Collagen/fibrinogen domain-containing protein 3, H-ficolin, Hakata antigen

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Sandwich ELISA - Human Ficolin-3 ELISA Kit (AB213779)
  • sELISA

Supplier Data

Sandwich ELISA - Human Ficolin-3 ELISA Kit (AB213779)

Human Ficolin-3 ELISA Kit (ab213779) Standard Curve.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Heparin Plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 20 pg/mL

검출 범위

0.78 - 50 ng/mL

분석 소요 시간

3h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

The Human Ficolin-3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213779) is designed for the quantitative measurement of Human Ficolin-3 in cell culture supernatants, serum and plasma (heparin).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-3 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: Q24-R299) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Ficolin-3 amount of sample captured in plate.

Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin (FN1), elastin (ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "25.739 ng/mL", "standardDeviation": "1.853", "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1.216 ng/mL", "standardDeviation": "0.08", "coefficientOfVariability": "= 6.6" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "6.253 ng/mL", "standardDeviation": "0.45", "coefficientOfVariability": "= 7.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "26.48 ng/mL", "standardDeviation": "1.403", "coefficientOfVariability": "= 5.3" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "5.741 ng/mL", "standardDeviation": "0.396", "coefficientOfVariability": "= 6.9" }, { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "1.219 ng/mL", "standardDeviation": "0.08", "coefficientOfVariability": "= 6.6" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FCN3 also known as ficolin-3 is a multifunctional protein recognized for its role in the immune system. Ficolin-3 is approximately 35 kDa in mass and is mainly expressed in the liver though it can also be found in various other tissues such as the spleen and lungs. It belongs to the ficolin family of proteins which are involved in innate immunity. The protein features a collagen-like domain and a fibrinogen-like domain facilitating its binding to sugars on microbial surfaces.
Biological function summary

Ficolin-3 acts as an essential player in the immune response contributing to pathogen recognition and clearance. It forms a complex with other components to activate the complement pathway particularly the lectin pathway. Ficolin-3 binds to carbohydrate patterns on the surface of pathogens leading to opsonization and clearance. This activity highlights its function as a defense mechanism against infections and its role in recognizing altered cells.

Pathways

Ficolin-3 facilitates the activation of the lectin complement pathway. This pathway is important for innate immune defense and involves several other proteins including mannose-binding lectin (MBL) and MASP-2 which are connected with ficolin-3 in mediating complement activation. The engagement of ficolin-3 in this pathway highlights its importance in identifying and eliminating pathogens effectively.

Mutations or deficiencies in the function of ficolin-3 are associated with an increased risk of infections and autoimmune diseases such as systemic lupus erythematosus (SLE). The protein's role in immune defense makes it a critical factor in maintaining a balanced immune response. Additionally related proteins like MBL can also influence the progression or predisposition to these conditions further emphasizing the interconnectedness of ficolin-3 within immune regulatory networks.
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제품 프로토콜

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타겟 정보

Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 11907111, PubMed : 15804047, PubMed : 17215869, PubMed : 26133042). Specifically recognizes and binds carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway (PubMed : 15804047, PubMed : 17215869, PubMed : 26133042). Specifically binds N-Acetylglucosamine (GlcNAc,) GalNAc (N-acetylgalactosamine) and D-fucose on the surface of pathogens, as well as mono/oligosaccharide and lipopolysaccharides from S.typhimurium and S.minnesota (PubMed : 17215869).
See full target information FCN3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine (Cambridge, Mass.) 27:129 PubMed34663207

2021

CXCL10 levels at hospital admission predict COVID-19 outcome: hierarchical assessment of 53 putative inflammatory biomarkers in an observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola I Lorè,Rebecca De Lorenzo,Paola M V Rancoita,Federica Cugnata,Alessandra Agresti,Francesco Benedetti,Marco E Bianchi,Chiara Bonini,Annalisa Capobianco,Caterina Conte,Angelo Corti,Roberto Furlan,Paola Mantegani,Norma Maugeri,Clara Sciorati,Fabio Saliu,Laura Silvestri,Cristina Tresoldi,Fabio Ciceri,Patrizia Rovere-Querini,Clelia Di Serio,Daniela M Cirillo,Angelo A Manfredi
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
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Product promise

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