AB322376

Human FKBP51 ELISA Kit- Extracellular

Recombinant
SimpleStep
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Human FKBP51 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human FKBP51 with a sensitivity of 44.069 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

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Sandwich ELISA - Human FKBP51 ELISA Kit- Extracellular (AB322376)

sELISA

Supplier Data

Sandwich ELISA - Human FKBP51 ELISA Kit- Extracellular (AB322376)

Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean FKBP51 concentration of detected samples was determined to be 164.6 pg/mL with a range of undetected – 406.3 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human FKBP51 ELISA Kit- Extracellular (AB322376)

Interpolated concentration of native FKBP51 was measured in duplicate at different sample concentrations. Undiluted samples are 25% HeLa cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human FKBP51 ELISA Kit- Extracellular (AB322376)

Example of human FKBP51 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human FKBP51 ELISA Kit- Extracellular (AB322376)

Example of human FKBP51 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 44.069 pg/mL

검출 범위

132.813 - 8500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human FKBP51 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FKBP51 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human FKBP51 with 44.069 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.5" } ]

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Recovery

[ { "sample": "Serum", "range": "85 - 95 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "84 - 85 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "90 - 102 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "90 - 96 %", "average": "= 92" }, { "sample": "Urine", "range": "88 - 102 %", "average": "= 94" } ]

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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab322376-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB322376-CMP10" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB322376-CMP08" }, { "size":"20 x 1 Vial", "name":"Human FKBP51 Lyophilized Recombinant Protein", "number":"AB322376-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human FKBP51 Detector Antibody", "number":"AB322376-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human FKBP51 Capture Antibody", "number":"AB322376-CMP01" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322376-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB322376-CMP07" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322376-CMP05" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322376-CMP09" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322376-CMP04" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab322376-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322376-CMP10" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB322376-CMP08" }, { "size":"2 x 1 Vial", "name":"Human FKBP51 Lyophilized Recombinant Protein", "number":"AB322376-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human FKBP51 Detector Antibody", "number":"AB322376-CMP02" }, { "size":"1 x 600 µL", "name":"10X Human FKBP51 Capture Antibody", "number":"AB322376-CMP01" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322376-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB322376-CMP07" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB322376-CMP11" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322376-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322376-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab322376-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB322376-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB322376-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human FKBP51 Lyophilized Recombinant Protein", "number":"AB322376-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FKBP51 Detector Antibody", "number":"AB322376-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human FKBP51 Capture Antibody", "number":"AB322376-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322376-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB322376-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322376-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB322376-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322376-CMP05", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Immunophilin protein with PPIase and co-chaperone activities (PubMed : 11350175). Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded (PubMed : 12538866). Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1 (PubMed : 28147277, PubMed : 28363942). Interacts with IKBKE and IKBKB which facilitates IKK complex assembly leading to increased IKBKE and IKBKB kinase activity, NF-kappa-B activation, and IFN production (PubMed : 26101251, PubMed : 31434731).

See full target information FKBP5

대체 명칭 보기

AIG6, FKBP51, FKBP5, Peptidyl-prolyl cis-trans isomerase FKBP5, PPIase FKBP5, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, Androgen-regulated protein 6, FF1 antigen, FK506-binding protein 5, FKBP54, HSP90-binding immunophilin, Rotamase, 51 kDa FKBP, FKBP-51, FKBP-5, p54

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human FKBP51 ELISA Kit- Extracellular

Sandwich ELISA - Human FKBP51 ELISA Kit- Extracellular (AB322376)

Sandwich ELISA - Human FKBP51 ELISA Kit- Extracellular (AB322376)

Sandwich ELISA - Human FKBP51 ELISA Kit- Extracellular (AB322376)

Sandwich ELISA - Human FKBP51 ELISA Kit- Extracellular (AB322376)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise