AB243677

Human GAD67/GAD1 ELISA Kit

Recombinant
SimpleStep
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Human GAD67/GAD1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GAD67/GAD1 with a sensitivity of 304.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

대체 명칭 보기

GAD, GAD67, GAD1, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, Glutamate decarboxylase 67 kDa isoform, GAD-67

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Sandwich ELISA - Human GAD67/GAD1 ELISA Kit (AB243677)

sELISA

Supplier Data

Sandwich ELISA - Human GAD67/GAD1 ELISA Kit (AB243677)

Example of human GAD standard curve in 1X Cell Extraction Buffer PTR.

The GAD standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human GAD67/GAD1 ELISA Kit (AB243677)

Representative image of Human GAD67/GAD1 ELISA Kit (ab243677)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human GAD67/GAD1 ELISA Kit Capture Antibody

- 10X Human GAD67/GAD1 Detector Antibody

- 10X Human GAD67/GAD1 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human GAD67/GAD1 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human GAD67/GAD1 ELISA Kit (AB243677)

Interpolated concentrations of native GAD in human brain tissue extract based on a 250 μg/mL extract load.

The concentrations of GAD were measured in duplicate and interpolated from the GAD standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GAD concentration was determined to be 4954 pg/mL in human brain tissue extract.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 304.3 pg/mL

검출 범위

781.3 - 50000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human GAD ELISA Kit (ab243677) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GAD protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human GAD with 304.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "104 - 107 %", "average": "= 106" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243677-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB243677-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243677-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB243677-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243677-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243677-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB243677-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243677-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243677-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB243677-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human GAD1/GAD67 Lyophilized Recombinant Protein", "number":"AB243677-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GAD1/GAD67 Capture Antibody", "number":"AB243677-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human GAD1/GAD67 Detector Antibody", "number":"AB243677-CMP09", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor.. Isoform 3. Enzymatically inactive as glutamate decarboxylase.

See full target information GAD1

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human GAD67/GAD1 ELISA Kit

Sandwich ELISA - Human GAD67/GAD1 ELISA Kit (AB243677)

Sandwich ELISA - Human GAD67/GAD1 ELISA Kit (AB243677)

Sandwich ELISA - Human GAD67/GAD1 ELISA Kit (AB243677)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Proteins & Peptides

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

Product promise