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AB269555

Human Galectin-3 ELISA Kit (LGALS3)

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(7 제품이 사용된 논문 )

Human Galectin-3 ELISA Kit (LGALS3) is a single-wash 90-min Simplestep used to quantify Human Galectin-3 (LGALS3) with a sensitivity of 13.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity- Cited in over 5 citations
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ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)
  • ELISA

Supplier Data

ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)
Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)

Interpolated concentrations of native Galectin-3 in human PBMC cell culture supernatant samples cultured for 36 hours.

The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 17.2 ng/mL in neat PBMC supernatant.

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)

Human Gelectin-3 standard curve comparison.

Standard Curve comparison between human Galectin-3 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)

Human Gelectin-3 standard curve comparison.

Standard Curve comparison between the original Human Gelectin-3 SimpleStep ELISA kit (ab188394) and current Human Gelectin-3 SimpleStep ELISA kit (ab269555).

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 40.4 ng/mL with a range of 27.2 – 66.0 ng/mL.

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)

Interpolated concentrations of native Galectin-3 in human MCF7 cell based on a 1.7 μg/mL extract load, Hap1 wild type and Galectin-3 knockout cells based on 200 µg/mL extract load.

The concentrations of Galectin-3 were measured in duplicate and interpolated from the Galectin-3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 1,855 pg/mL in MCF7 extract, 1,548 pg/mL in Hap1 wild type extract and undetectable in Hap1 Galectin-3 knockout extract.

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)

Interpolated concentrations of native Galectin-3 in human serum and plasma samples.

The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 6%, plasma (citrate) 6%, plasma (EDTA) 6% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 34.5 ng/mL in neat serum, 30.3 ng/mL in neat plasma (citrate), 29.9 ng/mL in neat plasma (EDTA) and 14.8 ng/mL in neat plasma (heparin).

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)
  • sELISA

Supplier Data

Sandwich ELISA - Human Galectin-3 ELISA Kit (LGALS3) (AB269555)

Example of human Galectin-3 standard curve in Sample Diluent NS.

The Galectin-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 13.3 pg/mL

검출 범위

58.8 - 2000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Galectin-3 ELISA Kit (LGALS3) ab269555 is a rapid single-wash 90-min Sandwich ELISA to measure Human Galectin-3 (LGALS3)in cell culture media, cell culture supernatant, cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 13.3 pg/mL.

How the assay works

Human Galectin-3 (LGALS3)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Galectin-3 ELISA Kit (LGALS3) ab269555 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is a member of the galectin family of carbohydrate-binding proteins. Galectin-3 is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is often pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "109 - 120 %", "average": "= 114" }, { "sample": "Serum", "range": "107 - 120 %", "average": "= 113" }, { "sample": "EDTA Plasma", "range": "111 - 116 %", "average": "= 113" }, { "sample": "Cell culture media", "range": "91 - 95 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "107 - 111 %", "average": "= 109" }, { "sample": "Cell Lysate", "range": "103 - 118 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "106 - 114 %", "average": "= 110" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like ?-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-?. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.
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제품 프로토콜

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타겟 정보

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus : acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
See full target information LGALS3

대체 명칭 보기

MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP
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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Life science alliance 8: PubMed40324823

2025

SLC38A9 is directly involved in Tat-induced endolysosome dysfunction and senescence in astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Neda Rezagholizadeh,Gaurav Datta,Wendie A Hasler,Erica C Nguon,Elise V Smokey,Nabab Khan,Xuesong Chen

Alzheimer's & dementia : the journal of the Alzheimer's Association 20:1515-1526 PubMed38018380

2023

Galectin-3 is upregulated in frontotemporal dementia patients with subtype specificity.

Applications

Unspecified application

Species

Unspecified reactive species

Sergi Borrego-Écija,Agnès Pérez-Millan,Anna Antonell,Laura Fort-Aznar,Elif Kaya-Tilki,Alberto León-Halcón,Albert Lladó,Laura Molina-Porcel,Mircea Balasa,Jordi Juncà-Parella,Javier Vitorica,Jose Luis Venero,Tomas Deierborg,Antonio Boza-Serrano,Raquel Sánchez-Valle

Experimental biology and medicine (Maywood, N.J.) 248:1383-1392 PubMed37787063

2023

Circulating H-FABP as a biomarker of frailty in patients with chronic heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Firdos Ahmad,Asima Karim,Javaidullah Khan,Rizwan Qaisar

Experimental biology and medicine (Maywood, N.J.) 248:532-540 PubMed36803120

2023

Plasma Galectin-3 and H-FABP correlate with poor physical performance in patients with congestive heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Firdos Ahmad,Asima Karim,Javaidullah Khan,Rizwan Qaisar

Biomolecules 12: PubMed36551191

2022

-GlcNAc-Mediated Regulation of Galectin Expression and Secretion in Human Promyelocytic HL-60 Cells Undergoing Neutrophilic Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Adam McTague,Rada Tazhitdinova,Alexander V Timoshenko

Acta neuropathologica 144:843-859 PubMed35895141

2022

Galectin-3 is elevated in CSF and is associated with Aβ deposits and tau aggregates in brain tissue in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Boza-Serrano,Agathe Vrillon,Karolina Minta,Agnes Paulus,Lluís Camprubí-Ferrer,Megg Garcia,Ulf Andreasson,Anna Antonell,Malin Wennström,Gunnar Gouras,Julien Dumurgier,Emmanuel Cognat,Laura Molina-Porcel,Mircea Balasa,Javier Vitorica,Raquel Sánchez-Valle,Claire Paquet,Jose Luis Venero,Kaj Blennow,Tomas Deierborg

Molecular psychiatry 26:7803-7812 PubMed34385598

2021

Angiogenic gene networks are dysregulated in opioid use disorder: evidence from multi-omics and imaging of postmortem human brain.

Applications

Unspecified application

Species

Unspecified reactive species

Emily F Mendez,Haichao Wei,Ruifeng Hu,Laura Stertz,Gabriel R Fries,Xizi Wu,Katherine E Najera,Michael D Monterey,Christie M Lincoln,Joo-Won Kim,Karla Moriel,Thomas D Meyer,Sudhakar Selvaraj,Antonio L Teixeira,Zhongming Zhao,Junqian Xu,Jiaqian Wu,Consuelo Walss-Bass
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