JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB309424

Human GLB1/Beta-galactosidase ELISA Kit

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Human GLB1/Beta-galactosidase ELISA Kit is a Sandwich ELISA for the measurement of Human GLB1/Beta-galactosidase in Human in Biofluids, Cell Culture Media samples.

대체 명칭 보기

ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase

1 이미지
Sandwich ELISA - Human GLB1/Beta-galactosidase ELISA Kit (AB309424)
  • sELISA

Supplier Data

Sandwich ELISA - Human GLB1/Beta-galactosidase ELISA Kit (AB309424)

Example of standard curve using ab309424. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.45 ng/mL

검출 범위

0.45 - 150 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Assay Kits

AB65351

Beta Galactosidase Staining Kit (Senescence)

Immunohistochemistry (Frozen sections) - Beta Galactosidase Staining Kit (Senescence) (AB65351)

  • 4
  • 1
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB197742

Mouse IL-1 beta ELISA Kit

Recombinant|SimpleStep

ELISA - Mouse IL-1 beta ELISA Kit (AB197742)

  • 5
  • 3
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Human GLB1/Beta-galactosidase ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CGLB1/Beta-galactosidase in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human GLB1/Beta-galactosidase coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "81 - 103 %", "average": "= 93.1" }, { "sample": "Plasma", "range": "96 - 108 %", "average": "= 102.5" }, { "sample": "Cell culture media", "range": "106 - 121 %", "average": "= 111.9" } ]
style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309424-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309424-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309424-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human GLB1/Beta-galactosidase Antibody-coated ELISA Plate", "number":"AB309424-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309424-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309424-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human GLB1/Beta-galactosidase Protein Standard", "number":"AB309424-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB309424-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human GLB1/Beta-galactosidase Detection Antibody", "number":"AB309424-CMP09", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GLB1 also known as beta-galactosidase is an enzyme that catalyzes the hydrolysis of beta-galactosides into monosaccharides. The protein has a molecular mass of approximately 76 kDa. Researchers observe this enzyme expression in the lysosome where it functions to break down complex carbohydrates. Its activity depends on proper glycosylation and correct folding usually occurring within specific cellular compartments.
Biological function summary

GLB1 is integral to the degradation and recycling of glycoproteins and glycolipids in the cell. It forms part of the lysosomal enzyme complex responsible for processing macromolecules. Without its function the accumulation of substrates can occur affecting cellular metabolism. The enzyme ensures that metabolic waste is efficiently processed linking GLB1 to cellular homeostasis.

Pathways

Beta-galactosidase participates in the lysosomal storage and glycan metabolism pathways. It is important for the catabolism of glycoside forms of hormones and other biologically-active molecules. In these pathways GLB1 interacts with proteins such as hexosaminidase creating a network of lysosomal enzymes. These interactions enable coordinated degradation of glycoproteins maintaining balance within cellular systems.

GLB1 mutations associate with conditions like GM1 gangliosidosis and Morquio syndrome type B. These are lysosomal storage disorders resulting from the accumulation of unmetabolized substrates. In these diseases hexosaminidase and other lysosomal enzymes show relationships with GLB1 illuminating complex interactions within cellular pathology. Therapeutic research aims to correct or enhance enzyme function seeking to alleviate disorder symptoms.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
See full target information GLB1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com