Human Granzyme A ELISA Kit
- Recombinant
- SimpleStep
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- sELISA
Supplier Data
Sandwich ELISA - Human Granzyme A ELISA Kit (AB255728)
Interpolated concentrations of native Granzyme A in human lung extract based on a 500 μg/mL extract load.
The concentrations of Granzyme A were measured in duplicate and interpolated from the Granzyme A standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
- sELISA
Supplier Data
Sandwich ELISA - Human Granzyme A ELISA Kit (AB255728)
Interpolated concentrations of native Granzyme A in human cell culture supernatant samples.
The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC stimulated supernatant 50% and Jurkat cell supernatant 50%. Human PBMCs and Jurkat cells were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were cultured for 2 days at 37°C in the presence or absence of 1.5% PHAM. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 2,017 pg/mL in PBMC stimulated supernatant and 1,644 pg/mL in Jurkat cell supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human Granzyme A ELISA Kit (AB255728)
Example of human Granzyme A standard curve in Sample Diluent NS.
The Granzyme A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Human Granzyme A ELISA Kit (ab255728) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Granzyme A protein in cell culture supernatant, edta plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Granzyme A with 6.61 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Granzyme A, also known as Cytotoxic T-lymphocyte proteinase 1, is a member of the granzyme family of serine proteases encoded by the gene GZMA. Granzyme A is released by cytoplasmic granules in cytotoxic T cells and Natural killer cells, where it can induce caspase-independent cell death. Granzyme A is generally more abundant than the other four granzyme proteins found in humans. Granzyme A is expressed as a precursor with a 26- aa signal peptide, a 2- aa pro-peptide and a 234- aa mature chain.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Functions of Granzyme A extend to inducing apoptosis in target cells alongside its role in promoting inflammation. It operates independent of caspases and directly cleaves substrates in the cell to induce cell death making it different from the related Granzyme B. As part of the granzyme-perforin complex it enters target cells via perforin-created pores mediates reactions leading to substrate degradation and promotes DNA damage.
Pathways
Granzyme A gets involved in the granule exocytosis pathway which constitutes an important mechanism for CTLs and NK cells to kill infected or cancerous cells. The protein is also linked to the inflammatory response pathways where it can interact with other serine proteases. Related proteins like perforin and Granzyme B also engage in these pathways and they collectively work to trigger apoptosis and modulate immune responses.
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