AB324502

Human Heme Oxygenase 2 (HO-2) ELISA Kit

SimpleStep
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Human Heme Oxygenase 2 (HO-2) ELISA Kit is a Sandwich ELISA for the measurement of Human Heme Oxygenase 2 (HO-2) in Human in Cell/Tissue Extracts samples.

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Sandwich ELISA - Human Heme Oxygenase 2 (HO-2) ELISA Kit (AB324502)

sELISA

Lab

Sandwich ELISA - Human Heme Oxygenase 2 (HO-2) ELISA Kit (AB324502)

Interpolated concentration of native Heme Oxygenase 2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 100 µg/mL HepG2 cell extract, 100 µg/mL HEK-293 cell extract, 200 µg/mL Liver tissue extract, and 500 µg/mL Kidney tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Human Heme Oxygenase 2 (HO-2) ELISA Kit (AB324502)

Example of human Heme Oxygenase 2 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Heme Oxygenase 2 (HO-2) ELISA Kit (AB324502)

Specificity and interference testing of human Heme Oxygenase 2 SimpleStep ELISA kit. 50 ng/mL of recombinant human Heme Oxygenase 1 (HO-1) was tested for specificity, and for interference in combination with 875 pg/mL of recombinant human Heme Oxygenase 2 (HO-2). The interpolated values, expressed in % of input (for specificity) or in % of human HO-2 control (for interference), are plotted (mean +/- SD, n = 2). No cross-reactivity or interference with HO-1 was observed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Extract, Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 18.269 pg/mL

검출 범위

54.688 - 3500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human Heme Oxygenase 2 (HO-2) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Heme Oxygenase 2 (HO-2) with a sensitivity of 18.269pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders.

-Design your own immunoassay: we also offer the conjugation-ready antibody pair.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.5" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "94 - 97 %", "average": "= 96" }, { "sample": "Tissue Extracts", "range": "91 - 105 %", "average": "= 96" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Heme oxygenase 2. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.. Heme oxygenase 2 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information HMOX2

대체 명칭 보기

HO2, HMOX2, Heme oxygenase 2, HO-2

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Heme Oxygenase 2 (HO-2) ELISA Kit

Sandwich ELISA - Human Heme Oxygenase 2 (HO-2) ELISA Kit (AB324502)

Sandwich ELISA - Human Heme Oxygenase 2 (HO-2) ELISA Kit (AB324502)

Sandwich ELISA - Human Heme Oxygenase 2 (HO-2) ELISA Kit (AB324502)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise