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AB219049

Human Hemoglobin subunit alpha ELISA Kit

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Human Hemoglobin subunit alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Hemoglobin subunit alpha with a sensitivity of 131 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)
  • sELISA

Supplier Data

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)

Interpolated concentrations of spike Hemoglobin subunit alpha in serum free cell culture media sample.

The concentrations of Hemoglobin subunit alpha were measured in duplicates, interpolated from the Hemoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : Serum free cell culture media 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)
  • sELISA

Supplier Data

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)

Interpolated concentrations of native Hemoglobin subunit alpha in human serum, plasma, and hemolyzed whole blood samples.

The concentrations of Hemoglobin subunit alpha were measured in duplicates, interpolated from the Hemoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (heparin) 25%, plasma (EDTA) 12.5%, and whole blood 1 : 80,000 . The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hemoglobin subunit alpha concentration was determined to be 0.07 μg/mL in serum, 0.06 μg/mL in plasma (heparin), 0.11 μg/mL in plasma (EDTA), and 400μg/mL in whole blood.

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)
  • sELISA

Supplier Data

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)

Interpolated concentrations of native Hemoglobin subunit alpha in human cerebral spinal fluid sample.

The concentrations of Hemoglobin subunit alpha were measured in duplicates, interpolated from the Hemoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : Cerebral spinal fluid 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hemoglobin subunit alpha concentration was determined to be 540 ng/mL in cerebral spinal fluid.

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)
  • sELISA

Supplier Data

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hemoglobin subunit alpha concentration was determined to be 34 ng/mL with a range of 8 – 137 ng/mL.

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)
  • sELISA

Supplier Data

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)

Interpolated concentrations of Natural Native Hemoglobin protein standard and hemoglobin subunit alpha recombinant protein.

Each protein was loaded at 5ng/mL and interpolated off the hemoglobin standard curve. The interpolated values are plotted (mean +/- SD, n=2). The observed 4-fold difference is expected, due to the size difference between the alpha subunit and the whole molecule.

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)
  • sELISA

Supplier Data

Sandwich ELISA - Human Hemoglobin subunit alpha ELISA Kit (AB219049)

Example of Human Hemoglobin subunit alpha standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Cerebral Spinal Fluid, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 131 pg/mL

검출 범위

588 - 20000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Hemoglobin subunit alpha ELISA Kit (ab219049) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hemoglobin subunit alpha protein in cerebral spinal fluid, cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Hemoglobin subunit alpha with 131 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Hemoglobin (Haemoglobin) subunits combine to form the quaternary structure of total hemoglobin, the oxygen carrying protein in red blood cells. In normal adults hemoglobin consists of 2 alpha and 2 beta subunits. Hemoglobin alpha subunit can be modified by the glucose on the terminal valine of beta subunits resulting in the Hemoglobin A1C molecule. The resulting A1C molecule is used to measure the average glucose levels in blood over time. ab219049 measures the total Hemoglobin and can recognize both the alpha subunit and A1C modified protein.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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Recovery

[ { "sample": "Serum", "range": "84 - 100 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "91 - 98 %", "average": "= 94" }, { "sample": "Cerebral Spinal Fluid", "range": "99 - 109 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "82 - 100 %", "average": "= 93" }, { "sample": "Whole Blood", "range": "81 - 115 %", "average": "= 101" }, { "sample": "serum free media", "range": "91 - 94 %", "average": "= 88" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin subunit alpha also known as alpha-globin is a component of the hemoglobin protein complex which plays an important role in oxygen transport within the blood. Alpha-globin has an approximate molecular weight of 15.1 kDa and is expressed highly in the red blood cells. It is part of the hemoglobin tetramer along with two beta subunits each one containing an iron-bound heme group. Variants of the alpha chain can be studied using hemoglobin peptides or denatured hemoglobin samples. Researchers can further analyze the alpha hemoglobin using methods like SDS-PAGE or alpha ELISA assays.
Biological function summary

Hemoglobin subunit alpha forms an important part of the hemoglobin complex facilitating the binding and release of oxygen molecules as blood circulates in the body. Alpha hemoglobin ensures efficient loading of oxygen in the lungs and unloading in tissues maintaining cellular respiration. The subunit plays a structural role as well stabilizing the hemoglobin tetramer for optimal function. Its ability to carry oxygen depends on the cooperative interaction between its alpha and beta globin counterparts.

Pathways

Hemoglobin subunit alpha operates predominantly within the oxygen transport pathway which is essential to meet the metabolic demands of cells. It also links to pathways involving iron metabolism given its coordination with heme groups. Alpha hemoglobin interacts cooperatively with proteins such as beta-globin to ensure efficient oxygen delivery. This interplay is highlighted when examining hemoglobin biosynthesis and breakdown pathways.

Alpha-globin is linked to conditions like alpha-thalassemia and sickle cell disease. These disorders result from mutations in the hemoglobin alpha or beta subunits leading to imbalanced globin production or abnormal hemoglobin structures. Alpha thalassemia is connected with unequal production of globin chains affecting hemoglobin stability while beta-globin mutations lead to sickle cell disease with altered oxygen delivery. Anti-hemoglobin antibodies might help in researching these conditions allowing a better understanding of molecular changes and potential therapeutic targets.
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제품 프로토콜

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타겟 정보

Involved in oxygen transport from the lung to the various peripheral tissues.. Hemopressin. Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1 (PubMed : 18077343). Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling (PubMed : 18077343).
See full target information HBA1

대체 명칭 보기

HBA2, HBA1, Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain
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websiteProtocolBooklet
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Product promise

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