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AB326895

Human Heparanase (HPSE) ELISA Kit, Chemiluminescent

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Human Heparanase (HPSE) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Heparanase (HPSE) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 18.29 - 40000 pg/ml with a sensitivity of 5.01 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
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Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)
  • sELISA

Lab

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)

Dynamic range comparison between the Chemiluminescent Human Heparanase SimpleStep ELISA and colorimetric Human Heparanase SimpleStep ELISA (ab256401). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)
  • sELISA

Lab

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)

Standard curve comparison between the Chemiluminescent Human Heparanase SimpleStep ELISA and colorimetric Human Heparanase SimpleStep ELISA (ab256401) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)
  • sELISA

Supplier Data

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)

Interpolated concentrations of native Heparanase in human spleen extract based on a 250 μg/mL extract load.

The concentrations of Heparanase were measured in duplicate and interpolated from the Heparanase standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heparanase concentration was determined to be 3,599 pg/mL in spleen extract.

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)
  • sELISA

Supplier Data

Sandwich ELISA - Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (AB326895)

Interpolated concentrations of native Heparanase in human serum and plasma samples.

The concentrations of Heparanase were measured in duplicates, interpolated from the Heparanase standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (citrate) 12.5%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heparanase concentration was determined to be 5,242 pg/mL in serum, 6,728 pg/mL in plasma (citrate) and 2,493 pg/mL in plasma (EDTA).

주요 정보

검출 방식

Luminescent

샘플 타입

Serum, EDTA Plasma, Tissue Extracts, Citrate plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 5.01 pg/mL

검출 범위

18.29 - 40000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (ab326895) is a rapid single-wash sandwich 55-min ELISA to measure Human Heparanase (HPSE) in Serum, EDTA Plasma, Tissue Extracts, Citrate plasma with a sensitivity of 5.01 pg/mL.

How the assay works

Human Heparanase (HPSE) ELISA Kit, Chemiluminescent (ab326895) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Heparanase (HPSE) (ab326895) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Heparanase antibody [EPR22365-202] - BSA and Aside free (Capture) ab259443
- Anti-Heparanase antibody [EPR22365-218] - BSA and Aside free (Detector) ab259444

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.5" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up-regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.
See full target information HPSE

대체 명칭 보기

HEP, HPA, HPA1, HPR1, HPSE1, HSE1, HPSE, Heparanase, Endo-glucoronidase, Heparanase-1, Hpa1
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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