Human HIF-2-alpha ELISA Kit, Chemiluminescent

• sELISA

Lab

Sandwich ELISA - Human HIF-2-alpha ELISA Kit, Chemiluminescent (AB326404)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

• sELISA

Lab

Sandwich ELISA - Human HIF-2-alpha ELISA Kit, Chemiluminescent (AB326404)

Dynamic range comparison between the Chemiluminescent Human HIF-2-alpha SimpleStep ELISA, colorimetric Human HIF-2-alpha SimpleStep ELISA (ab227898), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity.

• sELISA

Lab

Sandwich ELISA - Human HIF-2-alpha ELISA Kit, Chemiluminescent (AB326404)

Standard curve comparison between the Chemiluminescent Human HIF-2-alpha SimpleStep ELISA and colorimetric Human HIF-2-alpha SimpleStep ELISA (ab227898) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

• ELISA

Supplier Data

ELISA - Human HIF-2-alpha ELISA Kit, Chemiluminescent (AB326404)

Interpolated concentrations of native HIF-2-alpha in DFO treated Human HeL1 cell extract sample based on a 250 µg/mL extract load.

HELA cells were untreated or treated with 250 µM DFO for 24 hrs then collected and extracted according to sample preparation protocol (see section 11.1). The concentrations of HIF-2-alpha were measured in duplicate and interpolated from the HIF-2-alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean HIF-2-alpha concentration was determined to be 520 pg/mL in DFO treated HELA cell extract and undetectable in untreated HELA cell extract.

• ELISA

Supplier Data

ELISA - Human HIF-2-alpha ELISA Kit, Chemiluminescent (AB326404)

Dose-dependent induction of HIF-2-alpha in HELA cells by deferoxamine (DFO).

HeLa cells were cultured in 96-well tissue culture plates and were either untreated (Vehicle Control) or exposed to varying doses of DFO for 24 hours (DFO treated). Cells were extracted directly in the culture plate by overlaying culture media with Cell Extraction Buffer PTR with Enhancer such that the final concentration was 1X Cell Extraction Buffer PTR. Extracts were applied to the HIF-2-alpha SimpleStep ELISA plate. Raw data and standard deviation is plotted from quadruplicate measurements.