Human HNF-4-alpha ELISA Kit, Chemiluminescent

제품 세부 정보

Human HNF-4-alpha ELISA Kit, Chemiluminescent (ab325739) is a rapid single-wash sandwich 55-min ELISA to measure Human HNF-4-alpha in Cell Extract with a sensitivity of 2.02 pg/mL.

How the assay works

Human HNF-4-alpha ELISA Kit, Chemiluminescent (ab325739) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human HNF-4-alpha (ab325739) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-HNF-4-alpha antibody [EPR19265-107] - BSA and Aside free (Capture) ab242581
- Anti-HNF-4-alpha antibody [EPR19265-130] - BSA and Aside free (Detector) ab242827

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.