Human ICAM1 ELISA Kit (CD54)

• ELISA

Supplier Data

ELISA - Human ICAM1 ELISA Kit (CD54) (AB174445)

• ELISA

Supplier Data

ELISA - Human ICAM1 ELISA Kit (CD54) (AB174445)

Specificity of ICAM-1 signal on stimulated and non stimulated media supernatants.

Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were cultured for 2 days at 37⁰C in the presence or absence of PHA. The concentrations of ICAM-1 were interpolated from the calibration curve and corrected for sample dilution. The mean ICAM-1 concentration was determined to be 320 pg/mL in unstimulated PBMC supernatants and 2,654 pg/mL in stimulated PBMC supernatants.

• ELISA

Supplier Data

ELISA - Human ICAM1 ELISA Kit (CD54) (AB174445)

Frequency histogram of ICAM-1 levels in serum of individual normal healthy donors.

The levels of ICAM-1 in serum samples were tested from ten individual healthy donors. Levels were interpolated from the standard curve and corrected for sample dilution. The levels of ICAM-1 are shown for the percentage of individuals within each 100 ng/mL bin center of the distribution. The mean level of ICAM-1 was 469 ng/mL with a range of 347 to 629 ng/mL and a standard deviation of 77 ng/mL.

• ELISA

Supplier Data

ELISA - Human ICAM1 ELISA Kit (CD54) (AB174445)

Comparison of ICAM-1 levels in three Human cell culture lysates.

The levels of ICAM-1 protein were assessed in three Human cell line lysates loaded at 2.5 μg/mL of protein. The raw OD 450 nm signal for each sample was interpolated from an ICAM1 standard curve.

• sELISA

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Sandwich ELISA - Human ICAM1 ELISA Kit (CD54) (AB174445)

Example of ICAM-1 dynamic range in Raji cell lysates.

The curve was prepared by loading 5 μg/mL of Raji cell extracts, followed by a 1 : 2 titration series. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

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Sandwich ELISA - Human ICAM1 ELISA Kit (CD54) (AB174445)

Example ICAM-1 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

• ELISA

CiteAb

ELISA - Human ICAM1 ELISA Kit (CD54) (AB174445)

ELISA, Human ICAM1 ELISA Kit (CD54), ab174445. Publication image from Cashman, N. et al., 2017, Mol Neurodegener, 28830501. Legend direct from paper.

HDL suppresses Aβ-induced monocyte adhesion in engineered vessels via SR-BI. SR-BI blocking antibody was circulated through the lumen of engineered vessels prepared using HUVEC for 1 h prior treatment with 200 µg/mL of HDL for 2 h followed by injection of 1 µM of monomeric a Aβ40 or b Aβ42 within the tissue chamber (abluminal) for 8 h prior to injecting circulating fluorescent THP1 into the lumen. c-d ICAM-1 protein was measured in tissue lysates prepared in RIPA buffer by commercial ELISA. Graphs represent means ± SD of adhered monocytes relative to Aβ treated tissues from at least 4 individual grafts. * and # p < 0.05