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AB326437

Human IFN gamma ELISA Kit, Chemiluminescent

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Human IFN gamma ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IFN gamma with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 21.95 - 48000 pg/ml with a sensitivity of 4.05pg/mL.

-Patent pending
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Sandwich ELISA - Human IFN gamma ELISA Kit, Chemiluminescent (AB326437)
  • sELISA

Lab

Sandwich ELISA - Human IFN gamma ELISA Kit, Chemiluminescent (AB326437)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

ELISA - Human IFN gamma ELISA Kit, Chemiluminescent (AB326437)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit, Chemiluminescent (AB326437)

Interpolated concentrations of secreted IFNG in unstimulated and PHA-stimulated Human PBMC. The concentrations of IFNG were interpolated from data values using IFNG standard curve and corrected for sample dilution. The mean IFNG concentration was determined to be 1.8 ng/mL in unstimulated PBMC supernatants and 177.2 ng/mL in stimulated PBMC supernatants.

Sandwich ELISA - Human IFN gamma ELISA Kit, Chemiluminescent (AB326437)
  • sELISA

Lab

Sandwich ELISA - Human IFN gamma ELISA Kit, Chemiluminescent (AB326437)

Standard curve comparison between the Chemiluminescent Human IFN gamma SimpleStep ELISA and colorimetric Human IFN gamma SimpleStep ELISA (ab174443) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IFN gamma ELISA Kit, Chemiluminescent (AB326437)
  • sELISA

Lab

Sandwich ELISA - Human IFN gamma ELISA Kit, Chemiluminescent (AB326437)

Dynamic range comparison between the Chemiluminescent Human IFN gamma SimpleStep ELISA, colorimetric Human IFN gamma SimpleStep ELISA (ab174443), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

ELISA - Human IFN gamma ELISA Kit, Chemiluminescent (AB326437)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit, Chemiluminescent (AB326437)

Comparison of secreted IFNG in unstimulated and PHA-stimulated Human PBMC. PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 2 days. IFNG concentrations were measured in 12X and 6X diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC and media. Raw data values (mean +/-SD n=3) are graphed. The dotted line represents zero sample background.

주요 정보

검출 방식

Luminescent

샘플 타입

Cell culture media

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 4.05 pg/mL

검출 범위

21.95 - 48000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human IFN gamma ELISA Kit, Chemiluminescent (ab326437) is a rapid single-wash sandwich 55-min ELISA to measure Human IFN gamma in Cell culture media with a sensitivity of 4.05 pg/mL.

How the assay works

Human IFN gamma ELISA Kit, Chemiluminescent (ab326437) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IFN gamma (ab326437) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
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Recovery

[ { "sample": "Cell culture media", "range": "77 - 93 %", "average": "= 86" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG

대체 명칭 보기

Interferon gamma, IFN-gamma, Immune interferon, IFNG
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websiteProtocolBooklet
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Product promise

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