AB324327

Human IGF2R (M6PR) ELISA Kit

SimpleStep
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Human IGF2R (M6PR) ELISA Kit is a Sandwich ELISA for the measurement of Human IGF2R (M6PR) in Human in Biofluids, Cell Culture Media samples.

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Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean IGF2R concentration was determined to be 42.8 ng/mL with a range of 27.1 – 66.4 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

50 ng/mL each of recombinant human IGF2 was tested for specificity, and for interference in combination with 2,500 pg/mL of recombinant human IGF2R. The interpolated values, expressed in % of input (for specificity) or in % of human IGF2R control (for interference), are plotted (mean +/- SD, n =2).

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Example of human IGF2R standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Interpolated concentration of native IGF2R was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20% human plasma (EDTA). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Example of human IGF2R standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Interpolated concentration of native IGF2R was measured in duplicate at different sample concentrations. Undiluted samples are 20% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 64.904 pg/mL

검출 범위

156.25 - 10000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human IGF2R (M6PR) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGF2R (M6PR) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human IGF2R (M6PR) with 64.904 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.4" } ]

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Recovery

[ { "sample": "Serum", "range": "102 - 118 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "93 - 102 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "96 - 103 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "106 - 124 %", "average": "= 113" }, { "sample": "Cell culture media", "range": "97 - 103 %", "average": "= 101" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes (PubMed : 18817523, PubMed : 2963003). Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed : 18817523, PubMed : 2963003). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer (PubMed : 18817523). This receptor also binds IGF2 (PubMed : 18046459). Acts as a positive regulator of T-cell coactivation by binding DPP4 (PubMed : 10900005).

See full target information IGF2R

대체 명칭 보기

CD222, MPRI, IGF2R, Cation-independent mannose-6-phosphate receptor, CI Man-6-P receptor, CI-MPR, M6PR, 300 kDa mannose 6-phosphate receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, MPR 300, IGF-II receptor, M6P/IGF2R

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IGF2R (M6PR) ELISA Kit

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Primary Antibodies

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise