Human IGFBP1 ELISA Kit

• sELISA

Supplier Data

Sandwich ELISA - Human IGFBP1 ELISA Kit (AB233618)

Interpolated concentrations of native IGFBP1 in human milk and cell culture supernatant samples.

The concentrations of IGFBP1 were measured in duplicates, interpolated from the IGFBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 5%, A549 supernatant 100%, and HepG2 supernatant 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 12 ng/ml in milk, 0.5 ng/ml in A549 supernatant, and 172 ng/mL in HepG2 supernatant.

• sELISA

Supplier Data

Sandwich ELISA - Human IGFBP1 ELISA Kit (AB233618)

Interpolated concentrations of native IGFBP1 in human serum and plasma samples.

The concentrations of IGFBP1 were measured in duplicates, interpolated from the IGFBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 5%, plasma (heparin) 5%, and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 22 ng/mL in serum, 16 ng/mL in plasma (citrate), 21 ng/ml in plasma (heparin), and 37 ng/ml in plasma (EDTA).

• sELISA

Supplier Data

Sandwich ELISA - Human IGFBP1 ELISA Kit (AB233618)

Interpolated concentrations of native IGFBP1 in human HepG2, U-87 MG, and liver homogenate extract samples based on a 4 μg/mL, 500 μg/ml, and 500 μg/mL extract load respectively.

The concentrations of IGFBP1 were measured in duplicate and interpolated from the IGFBP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 580 pg/mL in HepG2 cell extract, 457 pg/ml in U-87 MG cell extract, and 739 pg/mL in liver tissue homogenate extract samples.

• sELISA

Supplier Data

Sandwich ELISA - Human IGFBP1 ELISA Kit (AB233618)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP1 concentration was determined to be 29.1 ng/mL with a range of 1.5 – 117.3 ng/mL.

• sELISA

Supplier Data

Sandwich ELISA - Human IGFBP1 ELISA Kit (AB233618)

Example of human IGFBP1 standard curve in 1X Cell Extraction Buffer PTR

• sELISA

Unknown

Sandwich ELISA - Human IGFBP1 ELISA Kit (AB233618)

Human IGFBP1 Competitor standard curve comparison.

Standard curve comparison between Human IGFBP1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

• sELISA

Supplier Data

Sandwich ELISA - Human IGFBP1 ELISA Kit (AB233618)

Example of human IGFBP1 standard curve in Sample Diluent NS.

The IGFBP1 standard curve was prepared as described in Section 10.