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AB314733

Human IkB alpha ELISA Kit

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Human IkB alpha ELISA Kit is a Sandwich ELISA for the measurement of Human IkB alpha in Human in Biofluids, Cell Culture Media samples.

대체 명칭 보기

IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha

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Sandwich ELISA - Human IkB alpha ELISA Kit (AB314733)
  • sELISA

Supplier Data

Sandwich ELISA - Human IkB alpha ELISA Kit (AB314733)

Example of standard curve using ab314733. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.65 ng/mL

검출 범위

0.65 - 150 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Primary Antibodies

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  • 31
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human IkB alpha ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IkB alpha in serum (human IkB alpha concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human IkB-alpha coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "84 - 103 %", "average": "= 97.18" }, { "sample": "Plasma", "range": "94 - 107 %", "average": "= 101.7" }, { "sample": "Cell culture media", "range": "119 - 145 %", "average": "= 133.3" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314733-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human IkB alpha Detection Antibody", "number":"AB314733-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314733-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314733-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human IkB alpha Protein Standard", "number":"AB314733-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314733-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314733-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human IkB alpha Antibody-coated ELISA Plate", "number":"AB314733-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB314733-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB314733-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed : 1493333, PubMed : 36651806, PubMed : 7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed : 7479976, PubMed : 7628694, PubMed : 7796813, PubMed : 7878466).
See full target information NFKBIA
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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