AB229436

Human IL-10 ELISA Kit, Fluorescent

CatchPoint SimpleStep ELISA
Recombinant
각 태그의 뜻

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Human IL-10 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human IL-10 with a sensitivity of 2.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

대체 명칭 보기

Interleukin-10, IL-10, Cytokine synthesis inhibitory factor, CSIF, IL10

3 이미지

Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (AB229436)

sELISA

Supplier Data

Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (AB229436)

Example of human IL-10 standard curve in Sample Diluent NS + Enhancer.

The IL-10 standard curve was prepared as described in Section 10. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (AB229436)

Human IL-10 Standard Curve Comparison.

Standard Curve comparison between human IL-10 current (new) SimpleStep ELISA kit and original ELISA kit. The current SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (AB229436)

Interpolated concentrations of native IL-10 in human PBMC cell culture supernatant samples.

The concentrations of IL-10 were measured in duplicates, interpolated from the IL-10 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10 concentration was determined to be 462 pg/ml in stimulated PBMC cell culture supernatant. PBMC cell culture supernatant samples were stimulated for 46 hours with 1.5% PHA. Unstimulated PBMC cell culture supernatant samples were also measured; all values were below the detectable range of the assay.

주요 정보

검출 방식

Fluorescent

샘플 타입

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.1 pg/mL

검출 범위

2.3 - 4800 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

IL-10 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 protein in human serum, plasma, and cell culture supernatant samples

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Interleukin 10 (IL-10) is a cytokine involved in immunoregulation through the promotion of Th2 response and inhibition of Th1 responses. It is produced by cells of hematopoietic origin, keratinocytes, placental cytotrophoblasts, and hepatic stellate cells.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "105 - 111 %", "average": "= 108" }, { "sample": "Serum", "range": "108 - 112 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "103 - 118 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "113 - 117 %", "average": "= 116" }, { "sample": "Citrate plasma", "range": "105 - 119 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "102 - 111 %", "average": "= 106" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab229436-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"500X Hydrogen Peroxide (H2O2, 3%)", "number":"AB229436-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stoplight Red Substrate Buffer", "number":"AB229436-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB229436-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB229436-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB229436-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI - HAMA Blocker (ab193969)", "number":"AB229436-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB229436-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent NBP", "number":"AB229436-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Stoplight Red Substrate", "number":"AB229436-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB229436-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-10 Capture Antibody", "number":"AB229436-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-10 Lyophilized Recombinant Protein", "number":"AB229436-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-10 Detector Antibody", "number":"AB229436-CMP04", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed : 16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed : 18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF (PubMed : 11564774, PubMed : 1940799, PubMed : 7512027). Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed : 8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

See full target information IL10

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

European journal of human genetics : EJHG 32:920-927 PubMed38605125

2024

Further evidence supporting the role of GTDC1 in glycine metabolism and neurodevelopmental disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Edoardo Errichiello,Mauro Lecca,Chiara Vantaggiato,Zoraide Motta,Nicoletta Zanotta,Claudio Zucca,Sara Bertuzzo,Luciano Piubelli,Loredano Pollegioni,Maria Clara Bonaglia

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-10 ELISA Kit, Fluorescent

Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (AB229436)

Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (AB229436)

Sandwich ELISA - Human IL-10 ELISA Kit, Fluorescent (AB229436)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise