AB324512

Human IL-11 ELISA Kit

SimpleStep
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Human IL-11 ELISA Kit is a Sandwich ELISA for the measurement of Human IL-11 in Human in Biofluids, Other samples samples.

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Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Interpolated concentration of native IL-11 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 15% treated MCR-5 cell culture supernatant (treated with 10 ng/mL PMA + 1 ng/mL IL-1 alpha for 48 hrs) and 60% untreated MCR-5 cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No signal was observed in neat cell culture media (DMEM containing 10% fetal bovine serum).

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Interpolated concentration of native IL-11 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : A549 cell culture supernatant 100%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Example of human IL-11 standard curve in 96-well vs. 384-well plate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Example of human IL-11 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Standard curve comparison between Human IL-11 SimpleStep ELISA kit (ab324512) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows comparable sensitivity.

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Standard curve comparison between the original Human IL-11 SimpleStep ELISA (ab189569) and current Human IL-11 SimpleStep ELISA (ab324512).

sELISA

Lab

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Interpolated concentration of native IL-11 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 15% treated MCR-5 cell culture supernatant (treated with 10 ng/mL PMA + 1 ng/mL IL-1 alpha for 48 hrs). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Citrate plasma, EDTA Plasma, Supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 17.38 pg/mL

검출 범위

43.75 - 2800 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human IL-11 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-11 with a sensitivity of 17.38pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Design your own immunoassay: we also offer the conjugation-ready antibody pair.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.1" } ]

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Recovery

[ { "sample": "Serum", "range": "93 - 100 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "88 - 110 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "83 - 85 %", "average": "= 84" }, { "sample": "Cell culture supernatant", "range": "93 - 99 %", "average": "= 96" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed : 2145578). Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation (PubMed : 12919066). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (PubMed : 30279168).

See full target information IL11

대체 명칭 보기

Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, IL11, IL 11, Interleukin 11

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-11 ELISA Kit

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

Sandwich ELISA - Human IL-11 ELISA Kit (AB324512)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise