AB223592

Human IL-12 p70 ELISA Kit

Recombinant
SimpleStep
각 태그의 뜻

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Human IL-12 p70 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-12 p70 with a sensitivity of 13.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

4 이미지

ELISA - Human IL-12 p70 ELISA Kit (AB223592)

ELISA

Supplier Data

ELISA - Human IL-12 p70 ELISA Kit (AB223592)

sELISA

Lab

Sandwich ELISA - Human IL-12 p70 ELISA Kit (AB223592)

Interpolated concentrations of spike IL-12 in human serum, plasma and cell culture supernatant samples.

The concentrations of IL-12 p70 were measured in duplicates, interpolated from the IL-12 p70 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%, plasma (citrate) 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Serum from ten individual healthy human female donors was measured in duplicate. No detectable levels of IL-12 p70 were measured.

sELISA

Lab

Sandwich ELISA - Human IL-12 p70 ELISA Kit (AB223592)

Example of human IL-12 p70 standard curve in 96-well vs. 384-well plate in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human IL-12 p70 ELISA Kit (AB223592)

Example of Human IL-12 standard curve.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 13.6 pg/mL

검출 범위

31.3 - 2000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human IL-12 p70 ELISA Kit ab223592 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-12 p70 in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 13.6 pg/mL.

How the assay works

Human IL-12 p70 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-12 p70 ELISA Kit ab223592 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

IL-12 p70 is a 70kDa cytokine heterodimer made of 2 subunits IL-12A (p35) and IL-12B (p40). This kit recognizes specifically the IL-12 total heterodimer protein. IL-12 p70 is produced by active antigen-presenting cells and promotes the development of Th1 and induces IFN?.

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Precision & recovery 정보

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" } ]

Recovery

[ { "sample": "Serum", "range": "93 - 101 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "97 - 101 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "80 - 89 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "92 - 94 %", "average": "= 93" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine (PubMed : 8605935, PubMed : 8943050). IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed : 1673147, PubMed : 1674604, PubMed : 8605935). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits (PubMed : 8943050). Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2 (PubMed : 7528775). In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (PubMed : 7638186). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and also functions as an immune-suppressive cytokine (By similarity). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers (PubMed : 22306691). Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (PubMed : 22306691).

See full target information IL12A

대체 명칭 보기

NKSF1, IL12A, Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, NK cell stimulatory factor chain 1, CLMF p35

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Fundamental & clinical pharmacology 38:958-966 PubMed39009501

2024

Bezafibrate mitigates oxidized-low density lipoprotein (ox-LDL)-induced the attachment of monocytes to endothelial cells: An implication in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Huijun Huang,Yan Shen

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Human IL-12 p70 ELISA Kit

ELISA - Human IL-12 p70 ELISA Kit (AB223592)

Sandwich ELISA - Human IL-12 p70 ELISA Kit (AB223592)

Sandwich ELISA - Human IL-12 p70 ELISA Kit (AB223592)

Sandwich ELISA - Human IL-12 p70 ELISA Kit (AB223592)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision & recovery 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise