AB303763

Human IL-13 receptor alpha 1 ELISA Kit

Recombinant
SimpleStep
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Human IL-13 receptor alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-13 receptor alpha 1 with a sensitivity of 5.939 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

4 이미지

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Interpolated concentrations of human IL-13 receptor alpha 1 in serum, plasma – citrate, plasma – EDTA, and plasma – heparin.

Interpolated concentration of native IL-13 receptor alpha 1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1 : 16 serum, 1 : 16 plasma – citrate, 1 : 16 plasma – EDTA, and 1 : 16 plasma – heparin. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Interpolated concentrations of human IL-13 receptor alpha 1 in HepG2 cell culture supernatant.

Interpolated concentration of native IL-13 receptor alpha 1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% for HepG2 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Example of human IL-13 receptor alpha 1 standard curve in Sample Diluent NS.

Example of human IL-13 receptor alpha 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Interpolated concentrations of IL-13 receptor alpha 1 in normal human serum donors.

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean IL-13 receptor alpha 1 concentration was determined to be 23.8 ng/mL with a range of 15.3 – 35.4 ng/mL

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 5.939 pg/mL

검출 범위

31.25 - 2000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Human IL-13 receptor alpha 1 SimpleStep ELISA® kit (ab303763) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-13 receptor alpha 1 protein in serum, plasma (cit), plasma (hep), plasma (EDTA) and cell culture supernatant. Quantitate Human IL-13 receptor alpha 1 with 5.939 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "109 - 117 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "84 - 100 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "93 - 110 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "82 - 113 %", "average": "= 100" }, { "sample": "Serum", "range": "90 - 113 %", "average": "= 105" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303763-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB303763-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB303763-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB303763-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303763-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303763-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303763-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303763-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-13 receptor alpha 1 Capture Antibody", "number":"AB303763-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-13 receptor alpha 1 Lyophilized Recombinant Protein", "number":"AB303763-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-13 receptor alpha 1 Detector Antibody", "number":"AB303763-CMP03", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.

See full target information IL13RA1

대체 명칭 보기

CD213a1, IL13R, IL13RA, IL13RA1, Interleukin-13 receptor subunit alpha-1, IL-13 receptor subunit alpha-1, IL-13R subunit alpha-1, IL-13R-alpha-1, IL-13RA1, Cancer/testis antigen 19, CT19

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-13 receptor alpha 1 ELISA Kit

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

Sandwich ELISA - Human IL-13 receptor alpha 1 ELISA Kit (AB303763)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise