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AB264618

Human IL-15RA ELISA Kit

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Human IL-15RA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-15RA with a sensitivity of 5.79 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)
  • sELISA

Lab

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Example of human IL-15RA standard curve in Sample Diluent NS + 1.5X Enhancer in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Interpolated concentrations of native IL-15RA in human serum and plasma.

The concentrations of IL-15RA were measured in duplicates, interpolated from the IL-15RA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-15RA concentration was determined to be 170.6 pg/mL in serum and 157.8 pg/mL in plasma (citrate).

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Interpolated concentrations of native IL-15RA in human cell culture supernatant samples.

The concentrations of IL-15RA were measured in duplicates, interpolated from the IL-15RA standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated PBMCs 50% and stimulated PBMCs 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-15RA concentration was determined to be undetectable in unstimulated PBMCs and 90.8 pg/mL in stimulated PBMCs.

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Representative image of Human IL-15RA ELISA Kit (ab264618)

Components shown from left to right :

- Sample diluent NS

- 10X Human IL-15RA Capture Antibody

- 10X Human IL-15RA Detector Antibody

- Human IL-15RA Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-15RA ELISA Kit (AB264618)

Example of human IL-15RA standard curve in Sample Diluent NS + 1.5X Enhancer.

The IL-15RA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Citrate plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 5.79 pg/mL

검출 범위

23.44 - 1500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human IL-15RA ELISA Kit (ab264618) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-15RA protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-15RA with 5.79 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin-15 receptor subunit alpha is one of the three subunits that is part of its hematopoietin receptor family. IL-15RA contributes to IL-15 mediated effects such as cell survival, cell proliferation, and activation of T and B lymphocytes. This kit recognizes extracellular fragment of IL-15RA, which is solubilized and binds to IL-15 with high affinity. IL-15 does not interfere in ab264618.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "81 - 96 %", "average": "= 90" }, { "sample": "Serum", "range": "107 - 121 %", "average": "= 115" }, { "sample": "Citrate plasma", "range": "96 - 121 %", "average": "= 110" } ]
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제품 구성

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab264618-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB264618-CMP10" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264618-CMP05" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB264618-CMP03" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB264618-CMP04" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264618-CMP01" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264618-CMP11" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264618-CMP06" }, { "size":"1 x 600 µL", "name":"10X Human IL-15RA Capture Antibody", "number":"AB264618-CMP08" }, { "size":"2 x 1 Vial", "name":"Human IL-15RA Lyophilized Recombinant Protein", "number":"AB264618-CMP07" }, { "size":"1 x 600 µL", "name":"10X Human IL-15RA Detector Antibody", "number":"AB264618-CMP09" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB264618-CMP12" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264618-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB264618-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264618-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264618-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264618-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264618-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264618-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264618-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB264618-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-15RA Lyophilized Recombinant Protein", "number":"AB264618-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-15RA Capture Antibody", "number":"AB264618-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-15RA Detector Antibody", "number":"AB264618-CMP09", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

High-affinity receptor for interleukin-15 (PubMed : 8530383). Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells (By similarity). In neutrophils, binds and activates kinase SYK in response to IL15 stimulation (PubMed : 15123770). In neutrophils, required for IL15-induced phagocytosis in a SYK-dependent manner (PubMed : 15123770). Expression of different isoforms may alter or interfere with signal transduction (PubMed : 10480910).. Isoform 5. Does not bind IL15.. Isoform 6. Does not bind IL15.. Isoform 7. Does not bind IL15.. Isoform 8. Does not bind IL15.
See full target information IL15RA

대체 명칭 보기

CD215, Interleukin-15 receptor subunit alpha, IL-15 receptor subunit alpha, IL-15R-alpha, IL-15RA, IL15RA
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websiteProtocolBooklet
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Product promise

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