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AB215539

Human IL-18 ELISA Kit

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(60 제품이 사용된 논문 )

Human IL-18 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-18 with a sensitivity of 8.3 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 40 citations
13 이미지
ELISA - Human IL-18 ELISA Kit (AB215539)
  • ELISA

Supplier Data

ELISA - Human IL-18 ELISA Kit (AB215539)
Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Interpolated concentrations of spike IL-18 in cell culture media and human urine samples.

The concentrations of IL-18 were measured in duplicates, interpolated from the IL-18 standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50% and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 concentration was determined to be 3,079 pg/mL in cell culture media and 4,119 pg/mL in urine.

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Interpolated concentrations of native IL-18 in human HeLa, HL-60, and A431 Cell Extract samples based on a 800 μg/ml extract load.

The concentrations of IL-18 were measured in duplicate and interpolated from the IL-18 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 concentration was determined to be 1,099 pg/ml in HeLa cell extract, 456 pg/ml in HL-60 cell extract and 567 pg/ml in A431 cell extract.

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Example of human IL-18 standard curve in Sample Diluent NS. The IL-18 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Example of human IL-18 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Serum from nine individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 concentration was determined to be 423 pg/mL with a range of undetectable – 2,852 pg/mL.

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Example of human IL-18 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Interpolated concentrations of spike IL-18 in human serum and plasma samples.

The concentrations of IL-18 were measured in duplicates, interpolated from the IL-18 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA), and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 concentration was determined to be 3,681 pg/mL in serum, 3,692 pg/mL in plasma (citrate), 6,941pg/mL in plasma (EDTA) and 4,057 pg/mL in plasma (heparin).

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Assay sensitivity.

The MDD was determined by calculating the mean of zero standard replicates and adding 2 standard deviations then extrapolating the corresponding concentration.

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native IL-18 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Linearity of dilution.

Recombinant IL-18 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Example of human IL-18 standard curve in 1X Cell Extraction Buffer PTR. The IL-18 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 ELISA Kit (AB215539)

Example of human IL-18 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 8.3 pg/mL

검출 범위

62.5 - 4000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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Primary Antibodies

AB263899

Anti-NLRP3 antibody [EPR23094-1]

KO Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-NLRP3 antibody [EPR23094-1] (AB263899)

  • 4
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human IL-18 ELISA kit ab215539 is a rapid single-wash 90-min sandwich ELISA kit to measure Human IL-18 in Urine, Cell culture extracts, Tissue Extracts, Plasma (Citrate, EDTA, Heparin) Cell culture supernatant, and Serum. This Simplestep sensitivity is 8.3 pg/mL.

How the assay works

Human IL-18 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Human IL-18 ELISA kit ab215539 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

How other researchers are using Human IL-18 ELISA kit ab215539

Human IL-18 ELISA kit ab215539 has been used to study melanoma(1), obesity (2), and age-related diseases (3).

References:

(1) Y. Zhang, et.al., 2023, PMID: 37620327
(2) S. Geng, et.al., 2023, PMID: 38156278
(3) B. Chhunchha, et.al., 2023, PMID: 38003466

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

Anti-IL-18 antibody [EPR19954-188] - BSA and Azide free (Capture) ab242590.
Anti-IL-18 antibody [EPR19956-19] - BSA and Azide free (Detector) ab242856

Related and Recommended products

Human IL-18 ELISA Kit ab215539 is commonly used to measure IL-18 as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Human IL-18BP ELISA Kit ab224877
- Human IL-37 ELISA Kit ab300313
- Human IRAK1 ELISA Kit ab219630
- Human IRAK4 ELISA Kit ab213472

Interleukin-18 (IL-18 or Interferon gamma-inducing factor) is a proinflammatory cytokine in the IL-1 superfamily. IL-18 has overlapping and synergistic biological function with IL-12. IL-18 is found in both intracellular and extracellular compartments. The antibodies in this kit were raised to the mature full-length sequence of human IL-18. Human IL-18 has 66% protein sequence identity to mouse Lipocalin-2.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision & recovery 정보

Precision

[ { "reproducibilityType": "Inter", "sample": "HeLa Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14.2" }, { "reproducibilityType": "Intra", "sample": "HeLa Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

Recovery

[ { "sample": "Serum", "range": "80 - 91 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "82 - 91 %", "average": "= 86" }, { "sample": "Cell culture extracts", "range": "93 - 101 %", "average": "= 96" }, { "sample": "Urine", "range": "86 - 91 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "91 - 97 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "80 - 84 %", "average": "= 82" }, { "sample": "Cell culture media", "range": "89 - 104 %", "average": "= 96" } ]

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses (PubMed : 10653850). Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators (PubMed : 14528293, PubMed : 25500532, PubMed : 37993714). Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells (PubMed : 10653850). Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed : 33883744).
See full target information IL18

대체 명칭 보기

IGIF, IL1F4, IL18, Interleukin-18, IL-18, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, IFN-gamma-inducing factor, IL-1 gamma
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제품이 사용된 논문 (60)

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