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AB113325

Human IL-18 R beta ELISA Kit

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Human IL-18 R beta ELISA Kit is a Sandwich ELISA for the measurement of Human IL-18 R beta in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human IL-18 R beta ELISA Kit (AB113325)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-18 R beta ELISA Kit (AB113325)

Representative Standard Curve using ab113325

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 12 pg/mL

검출 범위

12.3 - 3000 pg/mL

분석 플랫폼

Microplate

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보완 제품

Assay Kits

AB215539

Human IL-18 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-18 ELISA Kit (AB215539)

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Assay Kits

AB224877

Human IL-18BP ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-18BP ELISA Kit (AB224877)

  • 0
  • 0
Assay Kits

AB214025

Human IL-1 beta ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

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  • 1
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's IL-18 R beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-18 R beta in serum, plasma, cell culture supernatants. (Human IL-18 R beta concentration is pretty low in normal serum/plasma, it may not be detected in this assay)

This assay employs an antibody specific for Human IL-18 R beta coated on a 96- well plate. Standards and samples are pipetted into the wells and IL-18 R beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-18 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-18 R beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "68 - 85 %", "average": "= 75.78" }, { "sample": "Serum", "range": "71 - 89 %", "average": "= 80.9" }, { "sample": "Plasma", "range": "67 - 83 %", "average": "= 74.98" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113325-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113325-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113325-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB113325-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB113325-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IL-18 R beta Standard (lyophilized)", "number":"AB113325-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-18 R beta Microplate", "number":"AB113325-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IL-18 R beta", "number":"AB113325-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113325-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin Concentrate", "number":"AB113325-CMP04", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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