Human IL-25 ELISA Kit

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Human IL-25 ELISA kit (ab272200) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-25 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-25 with 41.8 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin-25, also known as IL-17E, is a cytokine that belongs to the IL-17 cytokine family and is encoded by the IL25 gene. IL-25 has a heterodimeric receptor composed of IL-17RA and IL-17RB, where both subunits are necessary for IL-25 functions and are expressed in the main Th2 cells. The main function of IL-25 is to regulate the adaptive immune responses by induction of several cytokines, such as; IL-4, IL-5, and IL-13. IL-25 supports the Th2 immune response and has been found to play a role in protecting against bowel infections by various intestinal parasites.