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AB238269

Human IL-28A ELISA Kit

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Human IL-28A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-28A with a sensitivity of 25.24 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

대체 명칭 보기

IL28A, ZCYTO20, IFNL2, Interferon lambda-2, IFN-lambda-2, Cytokine Zcyto20, Interleukin-28A, IL-28A

2 이미지
Sandwich ELISA - Human IL-28A ELISA Kit (AB238269)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-28A ELISA Kit (AB238269)

Interpolated concentrations of native IL28A in unstimulated and stimulated HUVEC cell culture supernatant samples.

HUVEC cells were stimulated by adding human IFN-a at 100 IU/mL for 20 hours, followed by the addition of Poly(I : C) at 30 μg/mL for 3 hours. The concentrations of IL28A were measured in duplicates, interpolated from the IL28A standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated HUVEC cell supernatant 100% and stimulated HUVEC cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). IL28A was not detectable in unstimulated HUVEC cell culture supernatant. The mean IL28A concentration was determined to be 545.5 pg/mL in stimulated HUVEC cell culture supernatant.

Sandwich ELISA - Human IL-28A ELISA Kit (AB238269)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-28A ELISA Kit (AB238269)

Example of human IL28A standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 25.24 pg/mL

검출 범위

98.44 - 6300 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human IL-28A ELISA Kit (ab238269) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-28A protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-28A with 25.24 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "HUVEC", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 101" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab238269-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB238269-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238269-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB238269-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB238269-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB238269-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB238269-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB238269-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-28A Lyophilized Recombinant Protein", "number":"AB238269-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-28A Capture Antibody", "number":"AB238269-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-28A Detector Antibody", "number":"AB238269-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information IFNL2
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websiteProtocolBooklet
en
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Product promise

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