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AB235637

Human IL-31 ELISA Kit

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Human IL-31 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-31 with a sensitivity of 29.83 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

대체 명칭 보기

Interleukin-31, IL-31, IL31

3 이미지
Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)

Interpolated concentrations of spike IL-31 in human serum, plasma (EDTA), and cell culture media (RPMI containing 10% FBS) samples.

The concentrations of IL-31 were measured in duplicates, interpolated from the IL-31 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (EDTA) 25%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)

Example of human IL-31 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)

Example of human IL-31 standard curve in Sample Diluent 50BS.

Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 29.83 pg/mL

검출 범위

117.2 - 7500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human IL-31 ELISA Kit (ab235637) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-31 protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-31 with 1.5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
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Recovery

[ { "sample": "Serum", "range": "98 - 102 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "92 - 98 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "103 - 109 %", "average": "= 105" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235637-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB235637-CMP12", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235637-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235637-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB235637-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB235637-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB235637-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235637-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB235637-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-31 Capture Antibody", "number":"AB235637-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-31 Lyophilized Recombinant Protein", "number":"AB235637-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-31 Detector Antibody", "number":"AB235637-CMP04", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR (PubMed : 15184896). May function in skin immunity (PubMed : 15184896). Enhances myeloid progenitor cell survival in vitro (By similarity). Induces RETNLA and serum amyloid A protein expression in macrophages (By similarity).
See full target information IL31
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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