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AB235640

Human IL-34 ELISA Kit

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Human IL-34 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-34 with a sensitivity of 3.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

대체 명칭 보기

C16orf77, IL34, Interleukin-34, IL-34

3 이미지
Sandwich ELISA - Human IL-34 ELISA Kit (AB235640)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-34 ELISA Kit (AB235640)

Competitor Curve Comparison.

Standard curve comparison between human IL-34 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human IL-34 ELISA Kit (AB235640)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-34 ELISA Kit (AB235640)

Interpolated concentrations of native IL-34 in human milk samples.

The concentrations of IL-34 were measured in duplicates, interpolated from the IL-34 standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 18.75%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-34 concentration was determined to be 1,014 pg/mL in neat human milk.

Sandwich ELISA - Human IL-34 ELISA Kit (AB235640)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-34 ELISA Kit (AB235640)

Example of human IL-34 standard curve in Sample Diluent 50BP.

The IL-34 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Saliva, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 3.4 pg/mL

검출 범위

28.1 - 1800 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human IL-34 ELISA Kit (ab235640) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-34 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-34 with 3.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Milk", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.6" }, { "reproducibilityType": "Intra", "sample": "Milk", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" } ]
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Recovery

[ { "sample": "Milk", "range": "111 - 115 %", "average": "= 113" }, { "sample": "Serum", "range": "81 - 88 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "89 - 96 %", "average": "= 92" }, { "sample": "Saliva", "range": "104 - 110 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "87 - 90 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "84 - 88 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "108 - 111 %", "average": "= 110" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235640-1x96Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB235640-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235640-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB235640-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB235640-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235640-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB235640-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235640-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BP", "number":"AB235640-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-34 Lyophilized Recombinant Protein", "number":"AB235640-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-34 Capture Antiibody", "number":"AB235640-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-34 Detector Antibody", "number":"AB235640-CMP05", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
See full target information IL34
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websiteProtocolBooklet
en
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Product promise

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