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AB46058

Human IL-4 ELISA Kit

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Human IL-4 ELISA Kit is a Sandwich ELISA for the measurement of Human IL-4 in Human in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Human IL-4 ELISA Kit (AB46058)
  • sELISA

Unknown

Sandwich ELISA - Human IL-4 ELISA Kit (AB46058)

Representative standard curve using ab46058

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 0.7 pg/mL

검출 범위

1.1 - 35 pg/mL

분석 소요 시간

3h 45m

분석 플랫폼

Microplate

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보완 제품

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's IL-4 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in supernatants, buffered solutions, serum and plasma samples.

A monoclonal antibody specific IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL4 present in the samples.

This kit will recognize both endogenous and recombinant Human IL-4.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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Recovery

[ { "sample": "Serum", "range": "75 - 121 %", "average": "= 100" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46058-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Control", "number":"AB46058-CMP10" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46058-CMP03" }, { "size":"1 x 1 Unit", "name":"IL-4 Microplate (12 x 8 well strips)", "number":"AB46058-CMP11" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46058-CMP01" }, { "size":"1 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46058-CMP08" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46058-CMP07" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46058-CMP06" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46058-CMP09" }, { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46058-CMP04" }, { "size":"2 x 1 Vial", "name":"IL-4 Standard (Lyophilized)", "number":"AB46058-CMP12" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46058-CMP05" }, { "size":"1 x 400 µL", "name":"Biotinylated anti IL-4", "number":"AB46058-CMP02" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46058-2x96Tests", "assetComponentDetails": [ { "size":"4 x 1 Vial", "name":"Control", "number":"AB46058-CMP10", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46058-CMP06", "productcode":"" }, { "size":"2 x 1 Unit", "name":"IL-4 Microplate (12 x 8 well strips)", "number":"AB46058-CMP11", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46058-CMP07", "productcode":"" }, { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46058-CMP04", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46058-CMP01", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46058-CMP09", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46058-CMP03", "productcode":"" }, { "size":"2 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46058-CMP08", "productcode":"" }, { "size":"4 x 1 Vial", "name":"IL-4 Standard (Lyophilized)", "number":"AB46058-CMP12", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46058-CMP05", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti IL-4", "number":"AB46058-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is also expressed on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).
See full target information IL4

대체 명칭 보기

Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of functional biomaterials 15: PubMed39194653

2024

Osteogenic Protection against Fine Dust with Erucic Acid-Induced Exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunjung Kim,Boyong Kim
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

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