JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB267599

Human IL36RN ELISA kit

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Human IL36RN ELISA kit is a Sandwich ELISA for the measurement of Human IL36RN in Human in Biofluids, Cell Culture Media samples.
1 이미지
Sandwich ELISA - Human IL36RN ELISA kit (AB267599)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL36RN ELISA kit (AB267599)

This standard curve is for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.07 ng/mL

검출 범위

0.069 - 50 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Assay Kits

AB219632

Human Thrombopoietin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Thrombopoietin ELISA Kit (AB219632)

  • 0
  • 0
Assay Kits

AB272200

Human IL-25 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-25 ELISA Kit (AB272200)

  • 0
  • 0
Assay Kits

AB216167

Human IL-17A ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-17A ELISA Kit (AB216167)

  • 0
  • 0
Proteins & Peptides

AB204926

Recombinant human IL-36RN protein

Bioactive

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Human IL36RN ELISA Kit is designed for the quantitative determination of IL36RN in cell culture supernatants, plasma and serum samples.

Δ Note: Human IL36RN concentration is low in normal serum/plasma and may not be detectable in this assay.

This assay employs an antibody specific for human IL36RN coated on a 96-well plate. Standards and samples are pipetted into the wells and IL36RN present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL36RN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL36RN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 RA (IL-1 F3), IL-18 (IL-1 F4), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-38 (IL-1 F10), IL-33 (IL-1 F11).

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "135 - 150 %", "average": "= 139.9" }, { "sample": "Plasma", "range": "111 - 145 %", "average": "= 129.1" }, { "sample": "Cell culture media", "range": "115 - 142 %", "average": "= 134.6" } ]
style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267599-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267599-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267599-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human IL36RN coated Microplate (12 x 8 wells)", "number":"AB267599-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267599-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267599-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB267599-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL36RN Standard (Lyophilized)", "number":"AB267599-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human IL36RN Detection Antibody", "number":"AB267599-CMP08", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
style
left-column
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2 and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus. May activate an anti-inflammatory signaling pathway by recruiting SIGIRR.
See full target information IL36RN

대체 명칭 보기

FIL1D, IL1F5, IL1HY1, IL1L1, IL1RP3, UNQ1896/PRO4342, IL36RN, Interleukin-36 receptor antagonist protein, IL-36Ra, FIL1 delta, IL-1-related protein 3, Interleukin-1 HY1, Interleukin-1 delta, Interleukin-1 family member 5, Interleukin-1 receptor antagonist homolog 1, Interleukin-1-like protein 1, IL-1RP3, IL-1HY1, IL-1 delta, IL-1F5, IL-1ra homolog 1, IL-1L1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com