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AB326391

Human IRAK-1 ELISA Kit, Chemiluminescent

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Human IRAK-1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IRAK-1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 18.29 - 40000 pg/ml with a sensitivity of 3.93pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending
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Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)
  • sELISA

Lab

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)
  • sELISA

Lab

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)

Dynamic range comparison between the Chemiluminescent Human IRAK-1 SimpleStep ELISA, colorimetric Human IRAK-1 SimpleStep ELISA (ab219630), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)
  • sELISA

Lab

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)

Standard curve comparison between the Chemiluminescent Human IRAK-1 SimpleStep ELISA and colorimetric Human IRAK-1 SimpleStep ELISA (ab219630) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)
  • sELISA

Supplier Data

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)

Interpolated concentrations of native IRAK-1 in human MCF7 HEK293T and K562 extracts based on a 500. 125 and 250 µg/mL extract load.

The concentrations of IRAK-1 were measured in duplicate and interpolated from the IRAK-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean IRAK-1 concentration was determined to be 8178 pg/mL in MCF7 extracts 3768 pg/mL in HEK93T extracts and 3927 pg/mL in K562 extracts.

주요 정보

검출 방식

Luminescent

샘플 타입

Cell culture extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 3.93 pg/mL

검출 범위

18.29 - 40000 pg/mL

분석 소요 시간

55m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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Primary Antibodies

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human IRAK-1 ELISA Kit, Chemiluminescent (ab326391) is a rapid single-wash sandwich 55-min ELISA to measure Human IRAK-1 in Cell culture extracts with a sensitivity of 3.93 pg/mL.

How the assay works

Human IRAK-1 ELISA Kit, Chemiluminescent (ab326391) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IRAK-1 (ab326391) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IRAK-1 antibody [EPR19667-168] - BSA and Azide free (Capture) ab242648
- Anti-IRAK-1 antibody [EPR19667-153] - BSA and Azide free (Detector) ab242893

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.3" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "107 - 113 %", "average": "= 110" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
See full target information IRAK1

대체 명칭 보기

IRAK, IRAK1, Interleukin-1 receptor-associated kinase 1, IRAK-1
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websiteProtocolBooklet
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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