JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB267651

Human Jagged1 ELISA Kit

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Human Jagged1 ELISA Kit is a Sandwich ELISA for the measurement of Human Jagged1 in Human in Biofluids, Cell Culture Media samples.
1 이미지
Sandwich ELISA - Human Jagged1 ELISA Kit (AB267651)
  • sELISA

Unknown

Sandwich ELISA - Human Jagged1 ELISA Kit (AB267651)

This standard curve is for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 1.6 ng/mL

검출 범위

1.638 - 400 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

style
left-column

보완 제품

Assay Kits

AB193765

Human alpha Fetoprotein ELISA Kit

Recombinant|SimpleStep

ELISA - Human alpha Fetoprotein ELISA Kit (AB193765)

  • 0
  • 0
Assay Kits

AB179887

Human Albumin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Albumin ELISA Kit (AB179887)

  • 3
  • 1
Assay Kits

AB239702

Bile Acid Assay Kit (Colorimetric)

Functional Studies - Bile Acid Assay Kit (Colorimetric) (AB239702)

  • 0
  • 0
Proteins & Peptides

AB108575

Recombinant human Jagged1 protein (Fc Chimera Active)

Bioactive

Functional Studies - Recombinant human Jagged1 protein (Fc Chimera Active) (AB108575)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Human Jagged1 ELISA Kit is designed for the quantitative determination of Jagged1 in cell culture supernatants, plasma and serum samples.

∆Note: Human Jagged1 concentration is low in normal serum/plasma, and may not be detectable in this assay.

This assay employs an antibody specific for human Jagged1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Jagged1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Jagged1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Jagged1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit detects human Jagged1. Other species not determined.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "69 - 90 %", "average": "= 77.24" }, { "sample": "Plasma", "range": "67 - 82 %", "average": "= 70.1" }, { "sample": "Cell culture media", "range": "75 - 127 %", "average": "= 99.66" } ]
style
left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267651-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267651-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267651-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Jagged1 coated Microplate (12 x 8 wells)", "number":"AB267651-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267651-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267651-CMP02", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB267651-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Jagged1 Standard (Lyophilized)", "number":"AB267651-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Jagged1 Detection Antibody", "number":"AB267651-CMP08", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Jagged1 also known as JAG1 or Jagged 1 protein is a transmembrane protein involved in the Notch signaling pathway. It has a mass of approximately 150 kDa and is expressed in various tissues including the cardiovascular system liver and mammary glands. Not only does it play a role in cell-cell communication but it also engages in controlling cell differentiation proliferation and apoptosis. Additionally researchers studying its molecular nature frequently employ Jagged1 ELISA kits to measure its expression and activity levels.
Biological function summary

The Jagged1 protein acts as a ligand binding to Notch receptors forming a large complex with other critical proteins within the pathway. This interaction initiates the signal transduction necessary for various cellular processes reinforcing the influence Jagged1 has on development and tissue homeostasis. Its role as a ligand amplifies its importance and scientists often use Jagged1 ELISA and Jag1 ELISA assays to explore these biological interactions further.

Pathways

Research shows that Jagged1 is an integral component of the Notch signaling and cardiovascular development pathways. It binds to Notch1 and Notch2 receptors facilitating important steps in both pathways which are related to embryonic development and adult tissue maintenance. This Notch-Jagged1 interaction also connects to Delta-like ligands creating an intricate network of proteins and pathways critical for a wide spectrum of cellular functions.

Jagged1 exhibits significant connections to Alagille syndrome and some cancers. Mutations in Jagged1 or disrupted interactions with Notch receptors often lead to the developmental defects characteristic of Alagille syndrome where both Jagged1 and Notch2 play significant roles. Additionally aberrant activation of the Notch signaling pathway involving Jagged1 has links to tumorigenesis in certain cancers opining avenues for potential therapeutic interventions against these diseases.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed : 18660822, PubMed : 20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed : 9462510). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
See full target information JAG1

대체 명칭 보기

CD339, JAGL1, JAG1, Protein jagged-1, Jagged1, hJ1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com