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AB288589

Human Kisspeptin ELISA Kit

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Human Kisspeptin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kisspeptin with a sensitivity of 5.472 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Human Kisspeptin ELISA Kit (AB288589)
  • sELISA

Supplier Data

Sandwich ELISA - Human Kisspeptin ELISA Kit (AB288589)

Interpolated concentration of native Kisspeptin was measured in duplicate at different sample concentrations. Undiluted samples are 250 μg/mL human placenta extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Kisspeptin ELISA Kit (AB288589)
  • sELISA

Supplier Data

Sandwich ELISA - Human Kisspeptin ELISA Kit (AB288589)

Example of human Kisspeptin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Tissue Extracts

Reacts with

Human

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 5.472 pg/mL

검출 범위

18.75 - 1200 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Secondary Antibodies

AB150116

Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)

Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (AB150116)

  • 5
  • 4
Primary Antibodies

AB275874

Anti-Kisspeptin antibody [EPR23770-189]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kisspeptin antibody [EPR23770-189] (AB275874)

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  • 0
Assay Kits

AB179884

Human Leptin ELISA Kit

SimpleStep

ELISA - Human Leptin ELISA Kit (AB179884)

  • 0
  • 0
Assay Kits

AB100533

Human hCG ELISA Kit

Sandwich ELISA - Human hCG ELISA Kit (AB100533)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human Kisspeptin ELISA Kit (ab288589) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Kisspeptin protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Kisspeptin with 5.472 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]
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Recovery

[ { "sample": "Tissue Extracts", "range": "95 - 110 %", "average": "= 104" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Kisspeptin also known as metastin is a peptide with significant roles in reproductive physiology. Found within the hypothalamus and several peripheral tissues it comprises a sequence of 54 amino acids and has a molecular mass of around 12.8 kDa. Kisspeptin binds to the G protein-coupled receptor GPR54 facilitating its regulatory functions. Widely expressed throughout the brain particularly in the arcuate nucleus and anteroventral periventricular nucleus it acts on neurons to influence GnRH secretion critically.
Biological function summary

Kisspeptin regulates the hypothalamic-pituitary-gonadal axis by promoting the release of gonadotropin-releasing hormone (GnRH). This stimulation enhances the synthesis and release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from the pituitary gland key components in reproductive maturation and fertility. Kisspeptin integrates into the neuroendocrine control network interacting closely with neurotransmitters and signaling molecules to maintain reproductive homeostasis.

Pathways

Kisspeptin plays an instrumental role in the reproductive signaling cascade. It serves as a critical upstream regulator in the GnRH signaling pathway essential for the control of puberty onset and reproductive function. The kisspeptin-GPR54 axis interacts with related proteins like neurokinin B and dynorphin which modulate its effect on GnRH neurons. These interactions create an intricate regulatory network essential to reproductive hormone feedback loops.

Altered kisspeptin signaling can contribute to conditions such as hypogonadotropic hypogonadism and polycystic ovary syndrome (PCOS). Mutations or deficiencies in the kisspeptin receptor GPR54 have been linked to hypogonadotropic hypogonadism impacting sexual development and fertility. PCOS involves complex kisspeptin-related pathways alongside insulin signaling dysregulation illustrating its intersection with metabolic pathways and potential treatment targets. Kisspeptin modulation affects significant pathways related to reproductive health making it a target of interest for therapeutic intervention in these conditions.
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제품 프로토콜

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타겟 정보

Kisspeptins are ligands for the G-protein coupled receptor KISS1R/GPR54 (PubMed : 11385580, PubMed : 11457843, PubMed : 11527393, PubMed : 12879005, PubMed : 15020672, PubMed : 15596153). The hypothalamic KISS1/KISS1R signaling system plays a central role in the regulation of the hypothalamic-pituitary-gonadal reproductive axis by modulating the secretion of gonadotropin-releasing hormone (GnRH) from GnRH neurons (PubMed : 15219839, PubMed : 15598687, PubMed : 22335740). In these neurons, kisspeptin binding to its receptor activates G(q)-dependent signaling, leading to phospholipase C (PLC) activation, and hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) (PubMed : 15598687, PubMed : 22335740). The subsequent rise in intracellular calcium levels results in the inhibition of inward rectifier potassium channels and activation of TRPC-like cation channels, leading to GnRH neurons depolarization and stimulation (By similarity). In addition to this pathway, kisspeptin also triggers G(q)-independent signaling via beta-arrestin, leading to MAPK cascade activation and ERK1/ERK2 phosphorylation (PubMed : 25147978). Kisspeptins are also involved in the regulation of other processes including cell growth, cell proliferation and cell migration (PubMed : 11385580, PubMed : 11457843, PubMed : 11527393, PubMed : 12879005, PubMed : 15020672, PubMed : 15596153).. Kisspeptin-10. Binds the G-protein coupled receptor KISS1R/GPR54 and triggers G protein-coupled receptor signaling via activation of G(q) and phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis by phospholipase C (PubMed : 11457843, PubMed : 22335740). Binding to the receptor also activates beta-arrestin-dependent signaling resulting in ERK1/2 phosphorylation (PubMed : 11457843, PubMed : 25147978). Activation of the receptor inhibits cell proliferation and cell migration, and is involved in the regulation of trophoblast invasion during early stages of pregnancy. Is also involved in the modulation of airway smooth muscle cells migration (PubMed : 11457843, PubMed : 15020672, PubMed : 38512807). In bone tissue, activation of KISS1R by kisspeptin-10 recruits phosphatase DUSP18 and SRC to the KISS1R C-terminus through a G(q)-dependent signaling pathway. This leads to DUSP18-mediated dephosphorylation of SRC, down-regulation of osteoclast differentiation and activity, and consequently suppression of bone resorption (By similarity).. Metastin. Receptor binding triggers G-protein coupled receptor signaling via activation of G(q) and phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolysis by phospholipase C.
See full target information KISS1

대체 명칭 보기

PP5098, KISS1, Metastasis-suppressor KiSS-1, Kisspeptin-1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70408 PubMed40098558

2025

Limosilactobacillus reuteri promotes the expression and secretion of enteroendocrine- and enterocyte-derived hormones.

Applications

Unspecified application

Species

Unspecified reactive species

Sara C Di Rienzi,Heather A Danhof,Micah D Forshee,Ari Roberts,Robert A Britton
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
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Product promise

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